Molecular phylogeny and species identification of pufferfish of the genus Takifugu (Tetraodontiformes, Tetraodontidae)
Molecular phylogeny and species identification of pufferfish of the genus Takifugu (Tetraodontiformes, Tetraodontidae)
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DOI：
10.1007/s10126-001-0006-5
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发表时间：
2001-07-01
影响因子：
3
通讯作者：
Qian, PY
中科院分区：
文献类型：
--
作者：
Song, LS;Liu, BZ;Qian, PY
利用随机扩增多态性DNA（RAPD）技术和线粒体16S核糖体RNA基因部分序列测定，对东方鲀属河豚的亲缘关系和种属鉴定进行了研究。在预定的最佳反应条件下，用200个10碱基引物扩增，产生1962个可重复的扩增片段，范围从200到3000 bp。根据扩增片段的有无，计算了5种东方鲀与作为外群的棕兔头鲀之间的遗传距离。使用通用引物扩增约572 bp的16S核糖体RNA基因，并用于确定遗传距离值。根据RAPD分析数据集和线粒体16SrDNA序列，采用邻接分析法构建了5种东方鲀及其外类群的拓扑进化树。红足东方鲀与假顶东方鲀之间的遗传距离与仙人掌种内个体间的遗传距离基本相同，但远小于红足东方鲀与假顶东方鲀之间的遗传距离。rubripes，T. pseudommus和其他物种。从线粒体和核DNA分析中收集的分子数据强烈表明T。rubripes和T. pseudommus应视为同一物种。从26株T. pseudommus个体和T. rubripes和T.假瘤在32 T。rubripes个体中，只有3个个体有扩增片段。这些结果表明，该片段可能有助于区分T。rubripes和T.假瘤
The phylogenetic relationships and species identification of pufferfishes of the genus Takifugu were examined by use of randomly amplified polymorphic DNA (RAPD) and sequencing of the amplified partial mitochondrial 16S ribosomal RNA genes. Amplifications with 200 ten-base primers under predetermined optimal reaction conditions yielded 1962 reproducible amplified fragments ranging from 200 to 3000 bp. Genetic distances between 5 species of Takifugu and Lagocephalus spadiceus as the outgroup were calculated from the presence or absence of the amplified fragments. Approximately 572 bp of the 16S ribosonial RNA gene was amplified, using universal primers, and used to determine the genetic distance values. Topological phylogenic trees for the 5 species of Takifugu and outgroup were generated from neighbor-joining analysis based on the data set of RAPD analysis and sequences of mitochondrial 16S rDNA. The genetic distance between Takifugu rubripes and Takifugu pseudommus was almost the same as that between individuals within cacti species, but much smaller than that between T. rubripes, T. pseudommus, and the other species. The molecular data gathered from both analysis of mitochondria and nuclear DNA strongly indicated that T. rubripes and T. pseudommus should be regarded as the same species. A fragment of approximately 900 bp was amplified from the genome of all 26 T. pseudommus individuals examined and 4 individuals of intermediate varieties between T. rubripes and T. pseudommus. Of the 32 T. rubripes individuals, only 3 had the amplified fragment. These results suggest that this fragment may be useful in distinguishing between T. rubripes and T. pseudommus.