Tridimensional structure of the Golgi apparatus of nonciliated epithelial cells of the ductuli efferentes in rat: an electron microscope stereoscopic study
Tridimensional structure of the Golgi apparatus of nonciliated epithelial cells of the ductuli efferentes in rat: an electron microscope stereoscopic study
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大鼠传出导管非纤毛上皮细胞高尔基体的三维结构：电子显微镜立体研究
作者：
A. Rambourg;Y. Clermont;L. Hermo;D. Segretain
用低、高压电子显微镜和体视法对大鼠输出管非纤毛上皮细胞薄片和厚片高尔基体的三维结构进行了分析。低倍镜下，高尔基体呈连续的网络状，在细胞核的顶端形成日冕。在高倍镜下，在组织切片中，用还原的臭氧后固定，柠檬酸铅染色或处理以显示磷酸酶活性，观察到以下结构特征。高尔基体网纵轴上有致密带和非致密带交替存在。致密带由6-8个扁平的、开窗较少的球囊组成，彼此紧密对接，形成堆叠。非致密区由多个高度开窗略膨大的球囊组成，这些球囊与致密区的球囊连续并连接在一起。在高尔基体的顺反轴上，可观察到以下隔室：(A)在顺式表面有一个连续的嗜欧小管网络，称为顺式元件；(B)一个顺式隔室，由3个或4个NADPase阳性球囊组成，球囊上穿孔，形成孔洞，形成小泡；(C)一个横隔室，由NADPase阳性元件下面的1或2个TPPAse阳性元件组成，后面是1或2个CMPase阳性元件，显示扁平的囊状部，与吻合管网络相连。这些管状网络从上面的元素弯曲离开，使这些元素具有“剥离”的结构。这些分子被称为球管分子，沿着高尔基体网络是不连续的。这个间隔还包括萎缩的跨肾小管网络，从上面的球囊小管分离出来，看起来正在碎裂成小泡和小管。横隔室元素的结构特征表明其持续更新。
The 3‐dimensional structure of the Golgi apparatus has been analyzed in thin and thick sections of nonciliated epithelial cells of ductuli efferentes of rat by use of low‐ and high‐voltage electron microscopes and a stereoscopic approach. In thick sections of tissue impregnated with osmium, the Golgi apparatus appeared at low magnification as a continuous network forming a corona at the apical pole of the nucleus. At higher magnification and in thin sections of tissue postfixed with reduced osmium and stained with lead citrate or treated to demonstrate phosphatase activity, the following structural features were observed. In the longitudinal axis of the Golgi network there were alternating compact and noncompact zones. The compact zones were composed of 6‐8 flattened, poorly fenestrated saccules in close apposition to each other and forming stacks. The noncompact zones were composed of a number of highly fenestrated and slightly distended saccules, which were continuous with and bridged the saccules of the compact zones. In the cis‐trans axis of the Golgi apparatus the following compartments were observed: (a) On the cis face there was a continuous osmiophilic tubular network referred to as the cis element; (b) a cis compartment composed of 3 or 4 NADPase‐positive saccules perforated with pores in register forming wells that contained small vesicles; (c) a trans compartment composed of 1 or 2 TPPAse‐positive elements underlying the NADPase ones, followed by 1 or 2 CMPase‐positive elements that showed a flattened saccular part continuous with a network of anastomotic tubules. These tubular networks curved away from the overlying elements, giving these elements a “peeling‐off” configuration. These elements referred to as sacculotubular elements were discontinuous along the Golgi network. This compartment also included shriveled trans‐tubular networks detached from the overlying sacculotubular elements and seemingly undergoing fragmentation into vesicles and tubules. The structural features of the elements of the trans compartment were indicative of continuous renewal.