Assessment of rat liver microsomal epoxide hydrolase as a marker of hepatocarcinogenesis.
Assessment of rat liver microsomal epoxide hydrolase as a marker of hepatocarcinogenesis.
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评估大鼠肝微粒体环氧化物水解酶作为肝癌发生的标志物。
DOI：
10.1016/0006-2952(85)90651-3
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发表时间：
1985
影响因子：
5.8
通讯作者：
M. Griffin
中科院分区：
文献类型：
--
作者：
D. Kizer;J. A. Clouse;D. Ringer;O. Hanson;A. Vaz;R. Palakodety;M. Griffin
测定了11种外源物质对肝微粒体环氧化物水解酶活性和含量的影响。用三种不同的底物对大鼠进行活性测定，连续3周，饲料中含有六种致癌物，即。2-乙酰氨基荧烷、3‘-甲基-4-二甲氨基偶氮苯、4’-氟-4-二甲氨基偶氮苯、硫代乙酰胺、黄曲霉毒素B1和乙硫氨酸。5种致癌物诱导活性为4-10倍；乙硫氨酸作为诱导剂效果相对较差。两种非致癌的肝癌类似物，即。菲和对氨基偶氮苯对酶活力无明显影响，而苯巴比妥、巴比妥钠和1-萘基异硫氰酸酯对酶活力有2~3倍的诱导作用。当使用自由基扩散法或酶联免疫吸附试验(ELISA)进行免疫化学检查时，所有11种异物都使微粒体环氧化物水解酶的数量增加了2-9倍。喂食10种外源化合物后，血清谷草转氨酶活性没有明显升高，表明诱导不是由毒性引起的。用ELISA法，在对照大鼠肝脏微粒体(PM)上清液中检测到微粒体环氧化物水解酶，从而证实了Gillet al早先的报道。[致癌3,1307(1982)]。这11种外源物质使PM上清液中可检测到的抗原量增加了3-34倍。对PM上清液进行较长时间的离心，得到的颗粒组分包含92±1.2%的ELISA可检测抗原，与异种方案无关。讨论了异种诱导微粒体环氧化物水解酶活性和量与肝癌发生的关系。
The influence of eleven xenobiotics on the activity and amount of hepatic microsomal epoxide hydrolase was determined. Activity was assayed using three different substrates after rats were fed, throughout 3 weeks, diets containing one of six hepatocarcinogens, viz. 2-acetylaminofluorene, 3′-methyl-4-dimethylaminoazobenzene, 4′-fluoro-4-dimethylaminoazobenzene, thioacetamide, aflatoxin B1and ethionine. Five hepatocarcinogens induced activity 4- to 10-fold; ethionine was relatively ineffective as an inducer. Two non-carcinogenic analogues of hepatocarcinogens, viz. fluorene andp-aminoazobenzene, caused no appreciable increase in enzyme activity, but phenobarbital, barbital and 1-naphthylisothiocyanate induced activity 2- to 3-fold. All eleven xenobiotics increased the amount of microsomal epoxide hydrolase 2- to 9-fold when examined immunochemically using either a radical diffusion assay or an enzyme-linked immunosorbent assay (ELISA). Serum glutamic oxaloacetic acid transaminase activity was not appreciably elevated by feeding ten of the xenobiotics, suggesting that inductions were not owing to toxicity. Using ELISA, microsomal epoxide hydrolase was detected in post-microsomal (PM) supernatant fractions from control rat liver, thus confirming an earlier report by Gillet al. [Carcinogenesis3, 1307 (1982)]. The eleven xenobiotics induced the amount of ELISA-detectable antigen in PM supernatant fractions by 3- to 34-fold. Longer centrifugation of PM supernatant fractions yielded a pellet fraction that contained 92 ± 1.2% of the ELISA-detectable antigen irrespective of the xenobiotic regimen. Relationships between xenobiotic induction of microsomal epoxide hydrolase activity and amount and hepatocarcinogenesis are discussed.
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表型多样性是肝癌发生过程中假定的癌前肝细胞群的早期特性。
影响因子：
11.2
作者：
Ogawa,K;Solt,DB;Farber,E
通讯作者：
Farber,E
大鼠增生性肝结节微粒体环氧化物水解酶的表征。
影响因子：
11.2
作者：
Oesch,F;Vogel-Bindel,U;Guenthner,TM;Cameron,R;Farber,E
通讯作者：
Farber,E
苯巴比妥、反式二苯乙烯氧化物和 3-甲基胆蒽对大鼠肝脏小叶中心、中带和门静脉周围区域环氧化物水解酶的影响。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kawabata,TT;Guengerich,FP;Baron,J
通讯作者：
Baron,J
实验性肝癌发生过程中环氧化物水解酶的免疫组织化学研究。
影响因子：
11.2
作者：
Enomoto,K;Ying,TS;Griffin,MJ;Farber,E
通讯作者：
Farber,E
DOI：
10.1111/j.1432-1033.1982.tb05894.x
发表时间：
1982
期刊：
European journal of biochemistry
影响因子：
--
作者：
Okada,Y;Frey,AB;Guenthner,TM;Oesch,F;Sabatini,DD;Kreibich,G
通讯作者：
Kreibich,G