Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2.
Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2.
复制标题
使用 Slide-SeqV2 识别和定位小鼠嗅球中的细胞类型。
DOI：
10.1007/978-1-0716-3425-7_13
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Yu,CRon
中科院分区：
文献类型：
--
作者：
Fang,Ai;Petentler,Kaitlyn;Price,Andrew;Malloy,Seth;Peterson,Michael;Maddera,Lucinda;Russell,Jonathon;Treese,McKenzie;Li,Hua;Wang,Yongfu;McKinney,Sean;Perera,Anoja;Yu,CRon
空间转录组学将RNA分子映射到它们在组织中表达的位置。在这里，我们记录了使用Slide-SeqV2来可视化小鼠嗅球（OB）中的基因表达。这种方法依赖于空间识别珠来定位和量化个体转录本。与珠相关的表达谱用于以公正的方式识别和定位单个细胞类型。我们展示了使用Slide-SeqV2识别嗅球中具有不同空间位置的各种细胞类型和亚型。
Spatial transcriptomics maps RNA molecules to the location in a tissue where they are expressed. Here we document the use of Slide-SeqV2 to visualize gene expression in the mouse olfactory bulb (OB). This approach relies on spatially identified beads to locate and quantify individual transcripts. The expression profiles associated with the beads are used to identify and localize individual cell types in an unbiased manner. We demonstrate the various cell types and subtypes with distinct spatial locations in the olfactory bulb that are identified using Slide-SeqV2.