Characterization of collagens synthesized by cultured bovine corneal endothelial cells.
Characterization of collagens synthesized by cultured bovine corneal endothelial cells.
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培养的牛角膜内皮细胞合成的胶原蛋白的表征。
DOI：
10.1016/s0021-9258(19)69615-8
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
R. Stern
中科院分区：
文献类型：
--
作者：
S. Tseng;N. Savion;D. Gospodarowicz;R. Stern
胶原蛋白是角膜内皮细胞培养产生的主要基质蛋白，占总蛋白合成的1%。使用已知的内部标记，通过DEAE-和CM-纤维素柱层析相结合的方法来确定不同类型的胶原。III型胶原是细胞外基质中沉积的主要成分，也是分泌到基质中的主要成分。基底膜的IV型和V型胶原也分布于每一个腔室，尽管后者优先与细胞基质联系。角膜内皮细胞合成的I：III：IV+V型胶原比例为3：16：1。
Collagen is the major matrix protein produced by corneal endothelial cell cultures and represented 1% of total protein synthesis. The different types of collagen were determined by a combination of DEAE- and CM-cellulose column chromatography using known internal markers. Type III collagen was the major component both deposited in the extracellular matrix and secreted into the media. The basement membrane collagens, types IV and V, were also found in each compartment, although the latter was associated preferentially with the cell matrix. The ratios of types I:III:IV + V collagens synthesized by corneal endothelial cells were 3:16:1.