Neptunomonas japonica sp nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan
Neptunomonas japonica sp nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan
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DOI：
10.1099/ijs.0.65509-0
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发表时间：
2008-04-01
影响因子：
2.8
通讯作者：
Horikoshi, Koki
中科院分区：
文献类型：
--
作者：
Miyazaki, Masayuki;Nogi, Yuichi;Horikoshi, Koki
从日本鹿儿岛附近抹香鲸尸体附近 226-246 m 深度的沉积物中分离出新的细菌种类。分离的菌株 JAMM 0745(T)、JAMM 1380、JAMM 1475 和 JAMM 1610 是革兰氏阴性、杆状、不形成孢子并通过单极或近末端鞭毛运动。基于新分离株的 16S rRNA 基因序列的系统发育分析表明，它们与多毛类 Osedax japonicus 的共生细菌克隆有关系（序列相似性为 99.6-99.9%），并且这些细菌与 Gammaproteobacteria 纲中的海王单胞菌属成员密切相关（相似性为 95.6-96.0%）。该新菌株能够产生类异戊二烯醌0-8作为主要醌成分。主要脂肪酸为 C-16:0、C-16:1 和 C-18:1，其中 C-18:2 和 C-20:2 含量较少。 4株新菌株的DNA G+C含量约为43.6-43.8 mol%。根据观察到的分类学差异，这四种分离菌株似乎代表了海王单胞菌属的新物种。学名 Neptunomonas japonica sp.十一月(型菌株JAMM 0745(T)=JCM 14595(T)=DSM 18939(T))被提议为新菌株。
Novel bacterial species were isolated from sediments adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 226-246 m. The isolated strains, JAMM 0745(T), JAMM 1380, JAMM 1475 and JAMM 1610, were Gram-negative, rod-shaped, non-spore-forming and motile by means of a single polar or subterminal flagellum. Phylogenetic analysis based on 16S rRNA gene sequences of the novel isolates indicated a relationship to a symbiotic bacterial clone of the polychaete Osedax japonicus (99.6-99.9 % sequence similarity) and these bacteria were closely related to members of the genus Neptunomonas (95.6-96.0% similarity) within the class Gammaproteobacteria. The novel strains were able to produce isoprenoid quinone 0-8 as the major quinone component. The predominant fatty acids were C-16:0, C-16:1 and C-18:1, with C-18:2 and C-20:2 present in smaller amounts. The DNA G + C contents of the four novel strains were about 43.6-43.8 mol%. Based on the taxonomic differences observed, the four isolated strains appear to represent a novel species of the genus Neptunomonas. The name Neptunomonas japonica sp. nov. (type strain JAMM 0745(T)=JCM 14595(T) =DSM 18939(T)) is proposed for the novel strains.