Effect of folic acid plus glycine supplement on uterine prostaglandin and endometrial granulocyte-macrophage colony-stimulating factor expression during early pregnancy in pigs
Effect of folic acid plus glycine supplement on uterine prostaglandin and endometrial granulocyte-macrophage colony-stimulating factor expression during early pregnancy in pigs
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DOI：
10.1016/s0093-691x(03)00213-9
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发表时间：
2004-01-15
期刊：
影响因子：
2.8
通讯作者：
Laforest, JP
中科院分区：
文献类型：
--
作者：
Guay, F;Matte, JJ;Laforest, JP
本研究旨在探讨叶酸+甘氨酸对初产母猪（NYL）、经产母猪（YL）和经产母猪（ML）子宫前列腺素代谢和子宫内膜粒细胞-巨噬细胞集落刺激因子（GM-CSF）mRNA表达的影响。在这三个组中，母猪被随机分配到两个处理组：15 ppm叶酸+0.6%甘氨酸或不补充。从交配前发情期至妊娠第25天屠宰期间给予膳食补充剂。屠宰时，采集子宫内膜组织，测定GM-CSF mRNA、环氧合酶-1（考克斯1）和-2（COX 2）的子宫内膜表达水平，并评价体外子宫内膜分泌前列腺素E-2（PGE（2））的分泌。同时收集尿囊液样品测定PGE（2）、前列腺素F-2 α的浓度。（PGF（2 α））、雌二醇-17 β（E-2）、孕酮（P-4）和转化生长因子-β（2）（TGF-β（2））。补充叶酸+甘氨酸对尿囊液中PGF（2 α）、E-2和P-4、P-3含量及子宫内膜体外分泌PGE（2）无明显影响。叶酸+甘氨酸添加剂有增加所有母猪尿囊液中PGE（2）和TGF-β（2）含量的趋势（P < 0.07），并增加子宫内膜COX 2的表达（P < 0.05），尤其是NYL母猪。叶酸+甘氨酸组子宫内膜COX 1表达水平显著降低（P < 0.05），尤以经产YL母猪为甚。尿囊液中PGE_2和PGF（2 α）含量不受母猪类型的影响。但NYL母猪子宫内膜体外分泌PGE（2，）和尿囊液中P4含量显著高于YL和ML母猪（P < 0.05）。
The objective was to determine the effects of folic acid+ glycine supplement on uterine metabolism of prostaglandin and mRNA expression of endometrial granulocyte-macrophage colony-stimulating factor (GM-CSF) in nulliparous (NYL) and multiparous Yorkshire-Landrace (YL) sows, and in multiparous Meishan-Landrace sows (ML). In each of these three groups, sows were randomly assigned to two treatments: 15 ppm folic acid + 0.6% glycine or no supplement. The dietary supplement was given from the estrus before mating to slaughter on Day 25 of pregnancy. At slaughter, endometrial tissue was collected to determine endometrial expression levels of GM-CSF mRNA, cyclooxygenase-1 (COX 1) and -2 (COX2) and to evaluate in vitro endometrial secretion of prostaglandin E-2 (PGE(2)) secretion. Allantoic fluid samples were also collected to determine the kconcentration of PGE(2), prostaglandin F-2alpha. (PGF(2alpha)), estradiol-17beta (E-2), progesterone( P-4), and transforming-growth factor-beta(2) (TGF-beta(2)). The allantoic contents of PGF(2alpha), E-2 and P-4,P- and endometrial in vitro secretion of PGE(2) were not significantly influenced by the folic acid + glycine supplement. The folic acid + glycine supplement tended (P < 0.07) to increase allantoic content of PGE(2) and TGF-beta(2) in all sows and increased (P < 0.05) endometrial expression of COX2, especially in NYL sows. The endometrial expression of COX1 was decreased (P < 0.05) by folic acid + glycine supplement, especially in multiparous YL sows. The allantoic contents of PGE2 and PGF(2alpha), were not significantly affected by sow type. However, NYL sows had higher (P < 0.05) endometrial in vitro secretion of PGE(2,) and allantoic content of P4 than multiparous YL and ML sows.