Protocol to refine intestinal motility test in mice
Protocol to refine intestinal motility test in mice
复制标题
DOI：
10.1258/002367704323133637
复制
发表时间：
2004-07-01
影响因子：
2.4
通讯作者：
Lucchesi, MBB
中科院分区：
文献类型：
--
作者：
Marona, HRN;Lucchesi, MBB
我们提出了一种新的方案，旨在符合3Rs(替换、减少和精炼)原则，使用禁食3h的动物来控制肠道运动，从而减少动物的应激。在这个新的方案中，小鼠被剥夺食物的时间很短(3小时)，并且不会被杀死。观察小鼠，直到观察到含有木炭的疏散，实验结果基于木炭疏散时间。本研究可能有助于制定可纳入关于小鼠禁食时间的修订指南的建议。这一肠道运动测试的新概念符合受人尊敬的科学。
We propose a new protocol intended to conform to the 3Rs (replacement, reduction, and refinement) principle, using animals fasted for 3 h to control intestinal motility, which reduced stress in the animals. In this new protocol, mice are deprived of food for a short time (3 h) and are not killed. The mice are observed until evacuation containing charcoal is observed, and the experimental results are based on the charcoal evacuation time. The present study may aid the formulation of recommendations that can be included in revised guidelines relating to the fasting time of mice. This new concept of an intestinal motility test conforms with respectful science.