Detection of cytomegalovirus (CMV) by real-time PCR in fecal samples for the non-invasive diagnosis of CMV intestinal disease
Detection of cytomegalovirus (CMV) by real-time PCR in fecal samples for the non-invasive diagnosis of CMV intestinal disease
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DOI：
10.1016/j.jcv.2014.10.009
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发表时间：
2014-12-01
影响因子：
8.8
通讯作者：
Heim, Albert
中科院分区：
文献类型：
--
作者：
Ganzenmueller, Tina;Kluba, Jeanette;Heim, Albert
背景资料：胃肠道巨细胞病毒（CMV）感染可引起CMV肠道疾病（CMV-ID），这是免疫功能低下患者的严重并发症。目前诊断CMV-ID的金标准需要分析结肠活检。CMV PCR检测粪便样本可能是一种非侵入性的诊断替代，但这种方法的数据是稀缺的。目的：评估使用定量CMV实时PCR粪便样本诊断CMV-ID. Study Design：粪便样本和下肠道活检66例进行了分析，定量CMV PCR。为了评价PCR检测粪便标本中CMV的诊断意义，根据肠道疾病的病因对患者进行分类结果：CMV-ID（根据内窥镜检查、组织病理学和活检中CMV DNA定量检测的结果）分为三组：“CMV-ID”、“非CMV-ID”和“可疑”。10/66份粪便样品通过定量CMV PCR检测为阳性，但CMV DNA载量较低（范围
Background: Cytomegalovirus (CMV) infection of the gastrointestinal tract can cause CMV intestinal disease (CMV-ID), a severe complication in immunocompromised patients. Current gold standard for diagnosing CMV-ID requires the analysis of colon biopsies. Testing of fecal samples by CMV PCR might be a non-invasive diagnostic alternative, but data on this method is scarce.Objectives: To evaluate the use of quantitative CMV real-time PCR in fecal samples for diagnosing CMV-ID. Study design: Fecal samples and lower intestinal tract biopsies from 66 patients were analyzed by quantitative CMV PCR. To evaluate the diagnostic significance of CMV detection by PCR in fecal samples, patients were classified according to the etiology of their intestinal disease (based on results of endoscopy, histopathology and quantitative CMV DNA detection in biopsies) into three groups: "CMV-ID", "non-CMV-ID", and "equivocal".Results: 10/66 fecal samples were tested positive by quantitative CMV PCR, but CMV DNA loads were low( range