Surface-enhanced raman scattering based nonfluorescent probe for multiplex DNA detection
Surface-enhanced raman scattering based nonfluorescent probe for multiplex DNA detection
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DOI：
10.1021/ac070078z
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发表时间：
2007-06-01
影响因子：
7.4
通讯作者：
Irudayaraj, Joseph
中科院分区：
文献类型：
--
作者：
Sun, Lan;Yu, Chenxu;Irudayaraj, Joseph
为了以简单、廉价和多重的方式快速准确地检测 DNA 标记，设计并制造了一种基于表面增强拉曼散射的替代探针，将 DNA 探测序列和非荧光拉曼标签共价连接到金纳米颗粒 (DNA-AuP-RTag) 的表面。探针拉曼信号的强度可以通过非荧光拉曼标签（RTag）的表面覆盖来控制。这些探针的检测灵敏度可以通过微调探针上 DNA 分子和 RTag 的数量来优化。 DNA-AuP-RTag 探针的长期稳定性良好（超过 3 个月）。通过同时鉴定混合物中多达八个探针，证明了 DNA-AuP-RTag 方案出色的多重检测能力。成功完成了单链 DNA 与其互补靶标杂交的检测，长期目标是在基于拉曼的 DNA 微阵列平台中使用非荧光 RTag。
To provide rapid and accurate detection of DNA markers in a straightforward, inexpensive, and multiplex format, an alternative surface-enhanced Raman scattering based probe was designed and fabricated to covalently attach both DNA probing sequence and nonfluorescent Raman tags to the surface of gold nanoparticles (DNA-AuP-RTag). The intensity of Raman signal of the probes could be controlled through the surface coverage of the nonfluorescent Raman tags (RTags). Detection sensitivity of these probes could be optimized by fine-tuning the amount of DNA molecules and RTags on the probes. Long-term stability of the DNA-AuP-RTag probes was found to be good (over 3 months). Excellent multiplexing capability of the DNA-AuP-RTag scheme was demonstrated by simultaneous identification of up to eight probes in a mixture. Detection of hybridization of single-stranded DNA to its complementary targets was successfully accomplished with a long-term goal to use nonfluorescent RTags in a Raman-based DNA microarray platform.