Quantification of Feline immunodeficiency virus (FIVpco) in peripheral blood mononuclear cells, lymph nodes and plasma of naturally infected cougars.
Quantification of Feline immunodeficiency virus (FIVpco) in peripheral blood mononuclear cells, lymph nodes and plasma of naturally infected cougars.
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自然感染美洲狮的外周血单核细胞、淋巴结和血浆中猫免疫缺陷病毒 (FIVpco) 的定量。
DOI：
10.1099/vir.0.81450-0
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Poss,Mary
中科院分区：
文献类型：
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作者：
Blake,DavidJ;Graham,Jon;Poss,Mary
家猫感染猫免疫缺陷病毒（FIV）可导致致命的免疫缺陷疾病，类似于人类免疫缺陷病毒1（HIV-1）。家猫血浆病毒载量升高与生存时间缩短和疾病进展相关。然而，FIV在猫科动物的其他成员中也保持为非致病性感染，包括美洲狮、美洲狮（FIVpco）。目前还不清楚美洲狮没有患病是否是病毒复制减少的结果。开发了一种实时PCR测定法，以定量自然感染的美洲狮中的HIV前病毒和血浆病毒载量。从外周血单个核细胞（PBMC）定量的前病毒载量范围为2.90 × 101 ~ 6.72 × 104拷贝/106细胞。血浆病毒载量范围为2.30 × 103至2.81 × 106 RNA拷贝ml−1。这些数据表明，FIVpcovirus载量与地方性和流行性慢病毒感染中观察到的病毒载量相当。因此，美洲狮没有疾病并不是因为病毒复制水平低。此外，在美洲狮PBMC前病毒载量之间观察到的显著差异与病毒谱系和美洲狮年龄相关（P= 0.014），这表明在FIVp谱系内存在单独的生活策略。这是第一个研究表明，慢病毒谱系和宿主年龄的相互作用显着影响前病毒载量。
Infection of domestic cats withFeline immunodeficiency virus(FIV) results in a fatal immunodeficiency disease, similar toHuman immunodeficiency virus 1(HIV-1) in humans. Elevated plasma viral loads in domestic cats are correlated to decreased survival time and disease progression. However, FIV is also maintained as an apathogenic infection in other members of the family Felidae including cougars,Puma concolor(FIVpco). It is not known whether the lack of disease in cougars is a result of diminished virus replication. A real-time PCR assay was developed to quantify both FIVpcoproviral and plasma viral loads in naturally infected cougars. Proviral loads quantified from peripheral blood mononuclear cells (PBMC) ranged from 2·90×101to 6·72×104copies per 106cells. Plasma viral loads ranged from 2·30×103to 2·81×106RNA copies ml−1. These data indicate that FIVpcoviral loads are comparable to viral loads observed in endemic and epidemic lentivirus infections. Thus, the lack of disease in cougars is not due to low levels of virus replication. Moreover, significant differences observed among cougar PBMC proviral loads correlated to viral lineage and cougar age (P=0·014), which suggests that separate life strategies exist within FIVpcolineages. This is the first study to demonstrate that an interaction of lentivirus lineage and host age significantly effect proviral loads.