EXCITATORY AMINO-ACID PROJECTIONS TO THE NUCLEUS-ACCUMBENS-SEPTI IN THE RAT - A RETROGRADE TRANSPORT STUDY UTILIZING D[H-3] ASPARTATE AND [H-3] GABA
EXCITATORY AMINO-ACID PROJECTIONS TO THE NUCLEUS-ACCUMBENS-SEPTI IN THE RAT - A RETROGRADE TRANSPORT STUDY UTILIZING D[H-3] ASPARTATE AND [H-3] GABA
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DOI：
10.1016/0306-4522(87)90345-9
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发表时间：
1987-08-01
期刊：
影响因子：
3.3
通讯作者：
BEART, PM
中科院分区：
文献类型：
--
作者：
CHRISTIE, MJ;SUMMERS, RJ;BEART, PM
已经通过注射 D[3H]天冬氨酸和逆行转运后的放射自显影术在大鼠中研究了利用谷氨酸或天冬氨酸对伏隔核的传入。采用[3H]GABA 体内注射的平行实验来确定 D[3H]天冬氨酸逆行标记的递质选择性性质。 D[3H]天冬氨酸标记的皮质和丘脑周核的形貌极其精确。 D[3H]天冬氨酸标记的核周体存在于无颗粒岛叶皮层V层；双侧在边缘前和边缘下分区 perikarya 内，但主要在同侧。在背侧、腹侧和后无颗粒岛叶皮质以及鼻周皮质中观察到同侧标记。注射到腹侧伏隔核中，标记同侧内嗅皮质中的核周，而将D[3H]天冬氨酸注入前尾壳核中，则导致同侧扣带回和外侧中央前皮质中的核周标记。输注 D[3H]天冬氨酸后，同侧中线丘脑核含有最高密度的标记核周体；以伏隔核为中心的输注导致带旁核的大量逆行标记，但侧向部位的标记稀疏，并且在注射到前尾壳核后未观察到。在其他丘脑核团（背内侧核、中央内侧核、菱形核、reuniens 核和中央外侧核）中观察到不太明显的周核标记，大部分位于中线附近。在同侧杏仁复合体中也发现了核周标记，特别是在基底外侧核和外侧核中。仅弱标记导致腹侧下托。前嗅核双侧存在大量标记细胞，但同侧核周细胞更为突出。在已知支配伏隔核的其他区域（腹侧被盖区、内侧黑质、中缝核和蓝斑）中并没有一致地观察到标记的核周。在腹侧苍白球/无名质、腹侧被盖区和内侧黑质中检测到推定顺行标记。 [3H]GABA 一般不会逆行转运至 D[3H]天冬氨酸标记的相同区域；前嗅核是一个例外，在那里发现了大量标记的周核。 [3H]GABA 未能标记丘脑和杏仁核中的核周，并且新皮质中不存在标记的地形分布。总的来说，我们的研究结果证实了 D[3H]天冬氨酸和 [3H]GABA 逆行转运的选择性，并提供了第一个证据，证明丘脑和杏仁核传入伏核利用谷氨酸和/或天冬氨酸作为其递质。他们还证实了之前的证据，即伏隔核的离皮质传入神经利用兴奋性氨基酸。以前从未描述过从前嗅核到伏核的传入。
Afferents to the nucleus accumbens septi utilizing glutamate or aspartate have been investigated in the rat by autoradiography following injection and retrograde transport of D[3H]aspartate. Parallel experiments with the intra-accumbal injection of [3H]GABA were employed to establish the transmitter-selective nature of the retrograde labelling found with D[3H]aspartate. The topography of cortical and thalamic perikarya labelled by D[3H]aspartate was extremely precise. D[3H]Aspartate labelled perikarya were found in layer V of agranular insular cortex; bilaterally within prelimbic and infralimbic subareas perikarya, but predominantly ipsilaterally. Ipsilateral labelling was observed in dorsal, ventral and posterior agranular insular cortices, and in perirhinal cortex. Injections into ventral accumbens labelled perikarya in ipsilateral entorhinal cortex, while infusion of D[3H]aspartate into anterior caudate-putamen resulted in labelling of perikarya in ipsilateral cingulate and lateral precentral cortices. Following infusion of D[3H]aspartate, ipsilateral midline thalamic nuclei contained the highest density of labelled perikarya; infusions centred on nucleus accumbens resulted in heavy retrograde labelling of the parataenial nucleus, but labelling was sparse from a lateral site and not observed after injection into anterior caudate-putamen. Less prominent labelling of perikarya was seen in other thalamic nuclei (mediodorsal, central medial, rhomboid, reuniens and centrolateral), mostly near the midline. Perikaryal labelling was also found in the ipsilateral amygdaloid complex, particularly in basolateral and lateral nuclei. Only weak labelling resulted in ventral subiculum. Numerous labelled cells were present bilaterally in anterior olfactory nucleus, although perikarya were more prominent ipsilaterally. Labelled perikarya were not consistently observed in other regions (ventral tegmental area, medial substantia nigra, raphe nuclei and locus coeruleus) known to innervate nucleus accumbens. Presumptive anterograde labelling was detected in ventral pallidum/substantia innominata, ventral tegmental area and medial substantia nigra. [3H]GABA was generally not retrogradely transported to the same regions labelled by D[3H]aspartate; an exception being the anterior olfactory nucleus, where large numbers of labeled perikarya were found. [3H]GABA failed to label perikarya in thalamus and amygdala, and a topographic distribution of label was absent in neocortex. Overall our findings confirm the selectivity of the retrograde transport of D[3H]aspartate and [3H]GABA, and provide the first evidence that thalamic and amygdaloid afferents to nucleus accumbens utilize glutamate and/or asparate as their transmitter. They also confirm previous evidence that corticofugal afferents to nucleus accumbens utilize an excitatory amino acid. Afferents from anterior olfactory nucleus to nucleus accumbens have not been previously described.