Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM
Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM
复制标题
DOI：
10.1016/j.vaccine.2009.04.010
复制
发表时间：
2009-06-12
期刊：
影响因子：
5.5
通讯作者：
Ogura, Hisashi
中科院分区：
文献类型：
--
作者：
Kato, Seiichi;Ohgimoto, Shinji;Ogura, Hisashi
CAM-70 麻疹病毒 (MV) 疫苗株目前用于预防麻疹疫苗接种。我们检查了 CAM-70 血凝素 (H) 蛋白的融合诱导能力，发现它在表达 CD46 和信号淋巴细胞激活分子 (SLAM) 的细胞中均受到损害。我们还产生了具有来自 CAM-70 菌株的 H 基因的重组 MV。 CAM-70 H 蛋白会损害表达 CD46 和 SLAM 的细胞中的病毒生长。在外周血淋巴细胞（PBL）和单核细胞衍生的树突状细胞（Mo-DC）中，CAM-70菌株不能有效生长。重组MV的感染表明，CAM-70菌株生长受损仅部分归因于PBL中的H基因，而大部分归因于Mo-DC中的H基因。因此，H蛋白融合诱导能力受损可能是导致CAM-70菌株减毒的潜在分子机制之一。 (C) 2009 Elsevier Ltd. 保留所有权利。
The CAM-70 measles Virus (MV) vaccine strain is Currently used for vaccination against measles. We examined the fusion-inducing ability of the CAM-70 hemagglutinin (H) protein and found that it was impaired in both CD46- and signaling lymphocyte activation molecule (SLAM)-expressing cells. We also generated recombinant MVs possessing H genes derived from the CAM-70 strain. The CAM-70 H protein impaired viral growth in both CD46- and SLAM-expressing cells. In peripheral blood lymphocytes (PBL) and monocyte-derived dendritic cells (Mo-DC), the CAM-70 strain did not grow efficiently. Infection with recombinant MVs revealed that impaired growth of the CAM-70 strain was attributed to the H gene only partly in PBL and largely in Mo-DC. Thus, impaired fusion-inducing ability of the H protein may be one of the underlying molecular mechanisms resulting in the attenuation of the CAM-70 strain. (C) 2009 Elsevier Ltd. All rights reserved.