Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF.
Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF.
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DOI：
10.1016/j.bbrc.2004.11.087
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发表时间：
2005-01
影响因子：
3.1
通讯作者：
Z. You;G. Duraine;J. L. Tien;Corinne A. Lee;T. Moseley;A. Reddi
中科院分区：
文献类型：
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作者：
Z. You;G. Duraine;J. L. Tien;Corinne A. Lee;T. Moseley;A. Reddi
白细胞介素-17 B（IL-17 B）是白细胞介素-17家族的成员，具有多种促炎和免疫调节活性。本研究发现IL-17 B mRNA在交配后14.5天的小鼠胚胎肢芽中表达最高，在交配后19.5天下降到低水平。免疫组织化学染色结果显示，IL-17 B在初级骨化中心的骨领细胞中表达最强。静止区和增殖区的软骨细胞呈中度染色，而肥大区几乎没有染色。在C3 H10 T1/2和MC 3 T3-E1细胞中，重组人骨形态发生蛋白-7上调IL-17 B mRNA表达，碱性成纤维细胞生长因子通过细胞外信号调节激酶途径下调IL-17 B mRNA表达。本研究首次证明IL-17 B在小鼠胚胎肢芽中表达，并可能在软骨形成和骨形成中发挥作用。
Interleukin-17B (IL-17B) is a member of interleukin-17 family that displays a variety of proinflammatory and immune modulatory activities. In this study, we found that IL-17B mRNA was maximally expressed in the limb buds of 14.5 days post coitus (dpc) mouse embryo and declined to low level at 19.5dpc. By immunohistochemical staining, the strongest IL-17B signals were observed in the cells of the bone collar in the primary ossification center. The chondrocytes in the resting and proliferative zones were stained moderately, while little staining was seen in the hypertrophic zone. Furthermore, in both C3H10T1/2 and MC3T3-E1 cells, the IL-17B mRNA was up-regulated by recombinant human bone morphogenetic protein-7, but down-regulated by basic fibroblast growth factor via the extracellular signal-regulated kinase pathway. This study provides the first evidence that IL-17B is expressed in the mouse embryonic limb buds and may play a role in chondrogenesis and osteogenesis.