Identification of protease substrates by combinatorial profiling on TentaGel beads
Identification of protease substrates by combinatorial profiling on TentaGel beads
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DOI：
10.1039/b713595e
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Reymond, Jean-Louis
中科院分区：
文献类型：
--
作者：
Kofoed, Jacob;Reymond, Jean-Louis
在TentaGel珠上与各种蛋白酶反应65 536个成员的八肽组合文库，然后在反应的珠表面对游离氨基末端进行选择性染色，并通过氨基酸分析进行序列测定，从而快速鉴定蛋白酶底物。
Reacting a 65 536 member combinatorial library of octapeptides on TentaGel beads with various proteases followed by selective staining of the free amino termini at the reacted bead surface and sequence determination by amino acid analysis allowed the rapid identification of protease substrates.