Effect of cytochrome b5 on the transbilayer distribution of phospholipids in model membranes.
Effect of cytochrome b5 on the transbilayer distribution of phospholipids in model membranes.
复制标题
细胞色素 b5 对模型膜中磷脂跨双层分布的影响。
DOI：
10.1021/bi00541a002
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Thompson,TE
中科院分区：
文献类型：
--
作者：
Nordlund,JR;Schmidt,CF;Holloway,PW;Thompson,TE
J. R. Nordlund， C. F. Schmidt， P. W. Holloway，和T. E. Thompson*摘要：在含有不同量细胞色素b的磷脂酰胆碱-磷脂酰乙醇胺囊泡中，测定了磷脂酰乙醇胺的跨双层分布。超声法制备的小泡和乙醇注射法制备的大泡经离心分离后，细胞色素b不对称地加入双分子层。磷脂与蛋白质的摩尔比在小囊泡中为280 ~ 560，在大囊泡中为100 ~ 500。在含有完整细胞色素b5分子的囊泡和含有完整细胞色素b5分子的囊泡中，用三硝基苯磺酸化学标记法测定了磷脂酰乙醇胺的分布
J. R. Nordlund, C. F. Schmidt, P. W. Holloway, and T. E. Thompson* abstract: The transbilayer distribution of phosphatidylethanolamine was assessed in phosphatidylcholine-phosphatidylethanolamine vesicles that contained various amounts of cytochrome b¡. The small vesicles, made by sonication, and the large vesicles, madeby ethanol injection, were fractionated by centrifugation before cytochrome b¡ was asymmetrically incorporated into the bilayer. The mole ratio of phospholipid to protein ranged from 280 to 560 in the small vesicles and from 100 to 500in the large vesicles. The phosphatidylethanolamine distribution, determined by chemical labeling with trinitrobenzenesulfonic acid, was assessed in vesicles that contained intact cytochrome b5 molecules and in vesicles where