Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae
Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae
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DOI：
10.1111/j.1365-2443.2005.00828.x
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发表时间：
2005-03-01
期刊：
影响因子：
2.1
通讯作者：
Kumagai, H
中科院分区：
文献类型：
--
作者：
Tamaki, H;Yun, CW;Kumagai, H
在酿酒酵母中，细胞大小受培养基中碳源种类的影响。在这里，我们提供的证据表明，Gpr1 受体和 Gpa2 Galpha 亚基是维持和调节细胞大小响应葡萄糖所必需的。在葡萄糖存在的情况下，缺乏 GPR1 或 GPA2 基因的突变体显示出比野生型菌株更小的细胞。生理学研究表明，突变菌株中蛋白质合成速率降低，表明生长速率降低，而所有菌株中 CLN1、2 和 3 的 mRNA 水平并未受到影响。基因芯片分析还揭示了突变菌株中与蛋白质和其他细胞成分生物合成相关的基因表达下调。我们还表明，GPR1 和 GPA2 是细胞大小响应葡萄糖而快速增加所必需的。通过添加葡萄糖，在乙醇中生长的野生型细胞的大小迅速增加，而在突变株中观察到的变化很小，其中由 CLN1 抑制引起的葡萄糖依赖性细胞周期停滞有所减轻。我们的研究表明，由 Gpr1 和 Gpa2 组成的酵母 G 蛋白偶联受体系统通过影响生长速率和细胞分裂来调节细胞大小。
In the yeast, Saccharomyces cerevisiae, cell size is affected by the kind of carbon source in the medium. Here, we present evidence that the Gpr1 receptor and Gpa2 Galpha subunit are required for both maintenance and modulation of cell size in response to glucose. In the presence of glucose, mutants lacking GPR1 or GPA2 gene showed smaller cells than the wild-type strain. Physiological studies revealed that protein synthesis rate was reduced in the mutant strains indicating that reduced growth rate, while the level of mRNAs for CLN1, 2 and 3 was not affected in all strains. Gene chip analysis also revealed a down-regulation in the expression of genes related to biosynthesis of not only protein but also other cellular component in the mutant strains. We also show that GPR1 and GPA2 are required for a rapid increase in cell size in response to glucose. Wild-type cells grown in ethanol quickly increased in size by addition of glucose, while little change was observed in the mutant strains, in which glucose-dependent cell cycle arrest caused by CLN1 repression was somewhat alleviated. Our study indicates that the yeast G-protein coupled receptor system consisting of Gpr1 and Gpa2 regulates cell size by affecting both growth rate and cell division.