Separation of isomeric disaccharides by traveling wave ion mobility mass spectrometry using CO2 as drift gas
Separation of isomeric disaccharides by traveling wave ion mobility mass spectrometry using CO2 as drift gas
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DOI：
10.1002/jms.3089
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发表时间：
2012-12-01
影响因子：
2.3
通讯作者：
Eberlin, Marcos N.
中科院分区：
文献类型：
--
作者：
Fasciotti, Maira;Sanvido, Gustavo B.;Eberlin, Marcos N.
使用CO2作为一个巨大的和可极化的漂移气体被证明大大提高峰-峰分辨率（RP-P），与N2相比，在突触G2行波离子迁移率细胞中的二糖的分离。近或基线RP-P实现了三对钠化分子的二糖异构体，即，纤维二糖和蔗糖（RP-P =？0.76）、麦芽糖和蔗糖（Rp-p =？1.04）、麦芽糖和乳糖（Rp-p =？0.74）。因此，使用CO2作为漂移气体的离子迁移率质谱法提供了一种有吸引力的替代品，用于快速有效地分离异构双糖。版权所有（c）2012约翰威利父子有限公司
The use of CO2 as a massive and polarizable drift gas is shown to greatly improve peak-to-peak resolution (Rp-p), as compared with N2, for the separation of disaccharides in a Synapt G2 traveling wave ion mobility cell. Near or baseline Rp-p was achieved for three pairs of sodiated molecules of disaccharide isomers, that is, cellobiose and sucrose (Rp-p?=?0.76), maltose and sucrose (Rp-p?=?1.04), and maltose and lactose (Rp-p?=?0.74). Ion mobility mass spectrometry using CO2 as the drift gas offers therefore an attractive alternative for fast and efficient separation of isomeric disaccharides. Copyright (c) 2012 John Wiley & Sons, Ltd.