A mammalian protein with specific demethylase activity for mCpG DNA
A mammalian protein with specific demethylase activity for mCpG DNA
复制标题
作者：
Bhattacharya, SK;Ramchandani, S;Szyf, M
DNA甲基化模式对于调节基因组功能是重要的，并且由甲基化和去甲基化的酶促过程决定。去甲基化酶现已被鉴定：哺乳动物互补DNA编码甲基-CpG结合结构域，具有将甲基化胞嘧啶碱基转化为胞嘧啶的去甲基化酶活性，并在体外将cDNA翻译或瞬时转染到人胚肾细胞中时使质粒去甲基化。这种DNA去甲基化酶的发现为DNA甲基化和去甲基化作用的分子和发育分析提供了基础。
DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.