Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein.
Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein.
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使用杂交瘤单克隆抗体检测狂犬病和狂犬病相关病毒蛋白之间的抗原差异。
DOI：
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发表时间：
1980
影响因子：
3.8
通讯作者：
H. Koprowski
中科院分区：
文献类型：
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作者：
A. Flamand;T. Wiktor;H. Koprowski
用狂犬病病毒或莫科拉病毒免疫的BALB/c小鼠的脾细胞与小鼠骨髓瘤细胞融合获得21株杂交瘤细胞，分泌针对诱导病毒核衣壳的单抗。它们对狂犬病病毒和狂犬病相关病毒的核衣壳表现出不同的特异性，可分为8个组，它们可能对应于核衣壳上不同的抗原决定簇。4株固定狂犬病病毒株(CVS、ERA、Flury-LEP和Kelev)不能被核衣壳特异性杂交瘤抗体区分。Flury-Hep病毒(来源于Flury-LEP)以及狂犬病相关病毒Mokola、Lagos Bat和Duvenhage与核衣壳杂交瘤抗体的反应性有显著差异。这些杂交瘤中的三个选定的小组可用于在LyssaVirus组的所有成员中快速鉴别诊断。
Twenty-one hybridoma cultures, obtained through the fusion of mouse myeloma cells with splenocytes of BALB/c mice immunized with either rabies virus or Mokola virus, secreted monoclonal antibodies specific for the nucleocapsid of the inducer virus. They displayed different specificities for the nucleocapsids of rabies and rabies-related viruses and could be classified into eight groups which are likely to correspond to different antigenic determinants on the nucleocapsid. Four strains of fixed rabies virus (CVS, ERA, Flury-LEP and Kelev) could not be differentiated by the nucleocapsid-specific hybridoma antibody. The Flury-HEP virus (derived from Flury-LEP) as well as the rabies-related viruses Mokola, Lagos bat and Duvenhage, showed marked differences in their reactivities with hybridoma antibodies to nucleocapsid. A selected panel of three of these hybridomas may be used for a rapid differential diagnosis among all members of the Lyssavirus group.