SELECTIVE KILLING OF NORMAL OR NEOPLASTIC B-CELLS BY ANTIBODIES COUPLED TO THE A-CHAIN OF RICIN
SELECTIVE KILLING OF NORMAL OR NEOPLASTIC B-CELLS BY ANTIBODIES COUPLED TO THE A-CHAIN OF RICIN
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DOI：
10.1073/pnas.77.9.5419
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
VITETTA, ES
中科院分区：
文献类型：
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作者：
KROLICK, KA;VILLEMEZ, C;VITETTA, ES
将针对正常或肿瘤鼠B淋巴细胞上的细胞表面IG的高度特异性抗体（亲和纯化或杂交瘤）共价偶联至植物毒素蓖麻毒素的A链。这种含有IgM、IgD的2种同种异型或B细胞肿瘤BCL 1的独特型特异性抗体的缀合物在体外快速结合表达相应表面抗原的细胞，并抑制这些细胞中的蛋白质合成。链偶联抗独特型抗体可用作肿瘤特异性细胞毒性剂。
Highly specific antibodies (affinity-purified or hybridoma) directed against cell surface Ig on normal or neoplastic murine B lymphocytes were covalently coupled to the A chain of the plant toxin ricin. Such conjugates containing antibodies specific for IgM, for either of the 2 allotypes of IgD, or for the idiotype of the B cell tumor BCL1 rapidly bound in vitro to cells expressing the corresponding surface antigen and inhibited protein synthesis in such cells. A chain-coupled anti-idiotype antibody may be useful as a tumor-specific cytotoxic agent.