UTILIZATION OF ADENOSINE-TRIPHOSPHATE IN RAT MAST-CELLS DURING HISTAMINE-RELEASE INDUCED BY ANAPHYLACTIC REACTION AND COMPOUND 48-80
UTILIZATION OF ADENOSINE-TRIPHOSPHATE IN RAT MAST-CELLS DURING HISTAMINE-RELEASE INDUCED BY ANAPHYLACTIC REACTION AND COMPOUND 48-80
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DOI：
10.1007/bf00500530
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发表时间：
1975-01-01
影响因子：
3.6
通讯作者：
CHAKRAVARTY, N
中科院分区：
文献类型：
--
作者：
JOHANSEN, T;CHAKRAVARTY, N
本文研究了大鼠腹膜肥大细胞三磷酸腺苷(ATP)含量与化合物48/80体外诱导组胺释放和抗原-抗体(过敏)反应的关系。当用寡霉素将活性致敏肥大细胞的ATP含量降低到不同程度时，其含量与过敏反应释放的组胺量之间有很好的相关性。先前已证实肥大细胞的ATP水平与化合物48/80诱导的组胺释放之间存在类似的线性关系。肥大细胞暴露于抗原或化合物48/80后不同时间段的ATP含量也进行了研究。在组胺释放的短时间内，未处理的肥大细胞的ATP含量没有明显变化。然而，如果用寡霉素或2-脱氧葡萄糖预先孵育肥大细胞以降低ATP合成速率，而大部分组胺释放不受影响，则ATP含量的降低可能与过敏性和化合物48/80诱导的组胺释放密切相关。观察表明，在释放过程中，肥大细胞对ATP的利用增加。
The ATP content of rat peritoneal mast cells has been studied in relation to histamine release induced by compound 48/80 and antigen-antibody (anaphylactic) reactionin vitro. When the ATP content of actively sensitized mast cells was reduced to different levels by oligomycin, a good correlation was obtained between the ATP levels and the amounts of histamine released by the anaphylactic reaction. A similar linear relation has previously been demonstrated between the ATP levels of mast cells and histamine release induced by compound 48/80. The ATP content of mast cells was also studied at different intervals after the exposure of the cells to antigen or compound 48/80. No significant change in the ATP content was observed in untreated mast cells during the short period when histamine release occurs. If, however, the mast cells were preincubated with oligomycin or 2-deoxyglucose to reduce the rate of ATP synthesis while a large part of the histamine release remained unaffected—a decrease in the ATP content could be demonstrated in close time relation to both anaphylactic and compound 48/80-induced histamine release. The observations indicate an increased utilization of ATP in mast cells during the release process.