Incorporation of hydroxyapatite into nanofibrous PLGA scaffold towards improved breast cancer cell behavior
Incorporation of hydroxyapatite into nanofibrous PLGA scaffold towards improved breast cancer cell behavior
复制标题
将羟基磷灰石纳入纳米纤维 PLGA 支架以改善乳腺癌细胞行为
DOI：
10.1016/j.matchemphys.2019.01.022
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发表时间：
2019-03-15
影响因子：
4.6
通讯作者：
Wan, Yizao
中科院分区：
文献类型：
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作者：
Luo, Honglin;Zhang, Yang;Wan, Yizao
癌细胞扩散到主要由羟基磷灰石（HAp）组成的骨骼组织可能表明癌细胞与HAp之间存在某种相关性。人们普遍认为，模拟体内微环境的有效实验模型（体外癌症模型）对于癌症研究非常重要。在此，首次将 HAp 纳米颗粒掺入聚乳酸-乙醇酸 (PLGA) 纳米纤维中，构建仿生 3D 纳米纤维支架作为体外癌症模型。对 PLGA/HAp 支架的形态、结构、机械和热性能进行了表征。所制备的 PLGA/HAp 支架表现出纤维直径减小、机械性能增强（拉伸强度和模量分别提高 31% 和 8%）以及比 PLGA 支架更粗糙的表面。以乳腺癌细胞系（MCF-7）为对照，评估PLGA/HAp支架的体外生物相容性，并通过流式细胞仪检测细胞周期进展。 MTT 测定和活体染色结果表明，与 PLGA 支架相比，PLGA/HAp 支架可以更好地支持细胞活力和增殖。细胞周期分析表明，DNA合成、细胞分裂和癌细胞增殖与PLGA纳米纤维中HAp的存在有关。我们的研究证明掺入 HAp 的 PLGA 纤维支架适合作为 3D 癌细胞培养的有前途的平台。
The spread of cancer cells to skeletal tissues which mainly consist of hydroxyapatite (HAp) may indicate some correlation between cancer cells and HAp. It is well accepted that an effective experimental model (in vitro cancer model) which mimics the microenvironment in vivo is of great importance for cancer research. Herein, for the first time, HAp nanoparticles are incorporated into poly(lactic-co-glycolic acid) (PLGA) nanofibers to construct biomimetic 3D nanofibrous scaffolds as an in vitro cancer model. The morphologies, structure, mechanical and thermal properties of the PLGA/HAp scaffolds were characterized. The as-prepared PLGA/HAp scaffolds exhibit decreased fiber diameter, enhanced mechanical properties (31% and 8% improvement in tensile strength and modulus, respectively), and rougher surface over PLGA scaffold. The in vitro biocompatibility of PLGA/HAp scaffolds was evaluated with breast cancer cell line (MCF-7) using PLGA scaffold as control and cell cycle progression was detected by flow cytometry. MTT assay and live staining results demonstrate that PLGA/HAp scaffolds can better support cell viability and proliferation over PLGA scaffold. Analysis of cell cycle reveals that DNA synthesis, cell division, and proliferation of cancer cell are related to the presence of HAp in PLGA nanofibers. Our studies demonstrate the suitability of HAp-incorporated PLGA fibrous scaffolds as a promising platform for 3D cancer cell culture.