Considerable rate of putative virulent phylo-groups in fecal carriage of extended-spectrum β-lactamase producing Escherichia coli
Considerable rate of putative virulent phylo-groups in fecal carriage of extended-spectrum β-lactamase producing Escherichia coli
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DOI：
10.1016/j.meegid.2019.04.035
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发表时间：
2019-09-01
影响因子：
3.2
通讯作者：
Shahcheraghi, Fereshteh
中科院分区：
文献类型：
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作者：
Aghamohammad, Shadi;Badmasti, Farzad;Shahcheraghi, Fereshteh
粪便携带产超广谱β-内酰胺酶肠杆菌目（ESBL-PE）已成为全球关注的健康问题。在无症状携带者中检测推定的产ESBL-EC强毒大肠杆菌（ESBL-EC）是公共卫生中的一个威胁性问题。本研究的目的是研究ESBL-EC在肠道的携带情况、ESBL-EC毒力群的分布以及ESBL-EC毒力群间的克隆相关性。共采集120份直肠拭子; 50.8%（61/120）来自重症监护室（ICU）住院患者，49.2%（59/120）来自门诊患者。用添加头孢噻肟的麦康凯琼脂进行ESBL-EC筛选。PCR法检测ESBL和碳青霉烯酶基因。采用接合试验、质粒复制子分型和多位点序列分型（MLST）等方法对致病菌群进行鉴定。120例受检者中ESBL-EC携带率为60.0%（72/120）。CTX-M-15、TEM和SHV的bla检出率分别为90.2%（65/72）、50.0%（36/72）和5.5%（4/72）。A、B1、B2、C、D和F组的出现率分别为20.8%（15/72）、6.9%（5/72）、20.8%（15/72）、2.7%（2/72）、13.8%（10/72）和12.5%（9/72）。在接合试验中，6个供试菌株中有5个带有接合质粒。最常见的质粒类型属于IncF不亲和组。MLST分析显示ESBL-EC分离株的主要序列类型为ST 769和ST 472。目前的研究提供了关于ESBL-EC分离株存在的新信息，特别是伊朗粪便携带者中假定的毒性大肠杆菌群。我们的数据显示，几乎有很高的ST之间的ESBL-EC菌株的异质性。为了实施有效的感染控制计划，建议在适当的时间（通常在入院初期）检测粪便携带情况。
Extended-Spectrum Beta-lactamase producing Enterobacteriales (ESBL-PE) in fecal carriage have become a global health concern. Detection of putative virulent ESBL-producing E.coli (ESBL-EC) isolates among asymptomatic carriers is a threatening issue in public health. The aim of this study was to investigate the intestinal carriage of ESBL-EC, phylo-groups and clonal relatedness among putative virulent groups of ESBL-EC isolated from fecal carriages. A total of 120 rectal swabs; 50.8% (61/120) from inpatients of intensive care unit (ICU) and 49.2% (59/120) from outpatients were collected. The ESBL-EC screening was performed by using MacConkey agar supplemented with cefotaxime. PCR assays were applied for determination of phylo-groups, detection of ESBL and carbapenemase genes. Conjugation experiment, plasmid replicon typing and Multilocus Sequence Typing (MLST) were performed for putative virulent phylo-groups. Totally, of 120 studied individuals, 60.0% (72/120) were carrier for ESBL-EC. The rate of bla(CTX-M-15), bla(TEM), bla(SHV) was 90.2% (65/72), 50.0% (36/72) and 5.5% (4/72), respectively. The frequency of phylo-groups A, B1, B2, C, D, and F were 20.8% (15/72), 6.9% (5/72), 20.8% (15/72), 2.7% (2/72), 13.8 (10/72) and 12.5% (9/72), respectively. In conjugation experiments, of 6 tested isolates, 5 had conjugative plasmids. The most prevalent plasmid types belonged to IncF incompatibility groups. The MLST analysis showed that the main sequence types among ESBL-EC isolates were ST769 and ST472. The current study provides novel information about the presence of the ESBL-EC isolates, particularly putative virulent phylo-groups among fecal carriages in Iran. Our data revealed that there was almost high ST heterogeneity among putative ESBL-EC isolates. In order to implementation of effective infection control program, detection of fecal carriage in appropriate time typically at the beginning of admission to the hospital is recommended.