JIP test for fast fluorescence transients as a rapid and sensitive technique in assessing the effectiveness of arbuscular mycorrhizal fungi in Zea mays: Analysis of chlorophyll a fluorescence
JIP test for fast fluorescence transients as a rapid and sensitive technique in assessing the effectiveness of arbuscular mycorrhizal fungi in Zea mays: Analysis of chlorophyll a fluorescence
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DOI：
10.1080/11263500802150225
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发表时间：
2008-07
期刊：
影响因子：
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通讯作者：
M. Rai;Sudhir S. Shende;R. Strasser
中科院分区：
文献类型：
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作者：
M. Rai;Sudhir S. Shende;R. Strasser
摘要利用叶绿素a荧光作为生物标志物，研究了菌根真菌印度梨形孢霉（Piriformospora indica）、摩西球囊霉（Glomus mosseae）和印度梨形孢霉（Piriformospora indica）、摩西球囊霉（Glomus mosseae）混合培养物对玉米植株生长的促进作用。 mosseae和G. caledonium），并将其与未接种的植物（对照）进行比较。色素系统II（Po）的量子效率没有受到太大的影响。电子流产量（Eo = Po *ψo）对印度稻和混合培养有很高的反应。内生菌对每个捕获中心的电子传递（ψo）有显著的影响。与未接种的植物相比，用印度稻和混合培养物处理的植物表现出相对较高的量子产量。每个反应中心的吸收量受内生菌的影响。印度梨形孢菌继之以混合接种物显示出与对照相比略低的值。形态学指标（株高和根系定殖率）反映了生物生理学指标（叶绿素a荧光），因此，JIP试验（一种用于分析快速荧光动力学O-J-I-P的试验）可以作为一种灵敏、快速的工具来测定菌根真菌在寄主植物中的效率。
Abstract We applied chlorophyll a fluorescence as a biomarker to assess the growth enhancement of maize plants after inoculation with the mycorrhizal fungi Piriformospora indica, Glomus mosseae, or a mixed culture (P. indica, G. mosseae and G. caledonium), and compared it with uninoculated plants (controls). The quantum efficiencies for pigment system II (ϕ Po) were not much affected. The yield for electron flow (ϕ Eo = ϕ Po *ψo) was highly responsive to P. indica and mixed culture. Electron transport per trapping center (ψo) was remarkably influenced by the endophytes. Plants treated with P. indica and mixed culture showed relatively higher quantum yield compared to uninoculated plants. Absorption per reaction center was influenced by the endophytes. Piriformospora indica followed by mixed inoculum showed slightly lower values as compared to controls. The morphological markers, namley height of the plants and percentage colonization in the roots, reflected the biophysiological markers (chlorophyll a fluorescence); hence, the JIP test (a test applied to analyze fast fluorescence kinetics O-J-I-P) can be used as a sensitive and rapid tool for determining the efficiency of mycorrhizal fungi in host plants.