Expression of uncoupling protein and alternative oxidase depends on lipid or carbohydrate substrates in thermogenic plants
Expression of uncoupling protein and alternative oxidase depends on lipid or carbohydrate substrates in thermogenic plants
复制标题
DOI：
10.1098/rsbl.2005.0338
复制
发表时间：
2005-12-22
期刊：
影响因子：
3.3
通讯作者：
Seymour, RS
中科院分区：
文献类型：
--
作者：
Ito, K;Seymour, RS
产热作用，其中细胞呼吸活性被大大刺激，需要线粒体解偶联蛋白（UCP）在哺乳动物和替代氧化酶（AOX）在植物中。在这里，我们表明，这两种蛋白质的基因在产热植物中表达，但类型与呼吸底物。一个新的基因命名为PsUCPa编码的UCP的变体，特异性地表达在喜林芋（Philodendron selloum）的产热花，它使用脂质作为底物。然而，一个名为DvAOX的基因编码的AOX蛋白表达的热生花的龙血竭，这可能是使用碳水化合物作为底物。这些结果表明，细胞代谢是一个主要的决定因素，在选择性表达适当的产热基因在植物中。
Thermogenesis, in which cellular respiratory activity is considerably stimulated, requires mitochondrial uncoupling protein (UCP) in mammals and an alternative oxidase (AOX) in plants. Here, we show that the genes for both proteins are expressed in thermogenic plants, but the type correlates with the respiratory substrate. A novel gene termed PsUCPa encoding a variant of UCP was specifically expressed in thermogenic flowers of Philodendron selloum, which uses lipids as substrates. However, a gene termed DvAOX encoding for AOX protein was expressed in thermogenic flowers of Dracunculus vulgaris, which presumably uses carbohydrates as substrates. These findings suggest that cellular metabolism is a major determinant in selective expression of appropriate thermogenic genes in plants.