MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain
MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain
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DOI：
10.1016/j.mcn.2008.07.016
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发表时间：
2008-10-29
影响因子：
3.5
通讯作者：
Saarma, Mart
中科院分区：
文献类型：
--
作者：
Lindholm, Paivi;Peranen, Johan;Saarma, Mart
中脑星形胶质细胞源性神经营养因子（MANF）已被描述为体外多巴胺能神经元的存活因子，但其在哺乳动物组织中的表达知之甚少。MANF与保守的多巴胺神经营养因子（CDNF）形成了一个新的进化保守的神经营养因子家族。在这里，我们使用原位杂交和免疫组织化学的特点MANF表达在发展和成年小鼠。MANF在神经系统和非神经元组织中广泛表达。在大脑中，在大脑皮层、海马和小脑浦肯野细胞中检测到相对较高的MANF水平。癫痫持续状态后，海马齿状回颗粒细胞层、丘脑网状核及部分皮质区Manf mRNA表达短暂增加。相反，全前脑缺血后Manf表达的变化在海马结构中广泛存在，在大脑皮层中更受限制。MANF的广泛表达及其进化保守性和脑损伤的调节表明，它在许多组织类型的正常和病理条件下都具有重要的功能。(C)2008年爱思唯尔公司All rights reserved.
The mesencephalic astrocyte-derived neurotrophic factor (MANF) has been described as a Survival factor for dopaminergic neurons in vitro, but its expression in mammalian tissues is poorly known. MANF and a homologous Protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. Here we used in situ hybridization and immunohistochemistry to characterize MANF expression in developing and adult mouse. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. After status epilepticus, Manf mRNA expression was transiently increased in the dentate granule cell layer of hippocampus, thalamic reticular nucleus and in several cortical areas. In contrast, following global forebrain ischemia changes in Manf expression were widespread in the hippocampal formation and more restricted in cerebral cortex. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types. (C) 2008 Elsevier Inc. All rights reserved.