Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adipose-derived mesenchymal cells
Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adipose-derived mesenchymal cells
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DOI：
10.1016/j.bbrc.2006.02.171
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发表时间：
2006-05-05
影响因子：
3.1
通讯作者：
Longaker, MT
中科院分区：
文献类型：
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作者：
Chiou, M;Xu, Y;Longaker, MT
脂肪来源的间充质细胞（AMC）已被证明具有向骨、软骨和脂肪分化的巨大能力。利用骨髓间充质干细胞（BMSCs）的研究表明，成纤维细胞生长因子（FGF）-2是一种强有力的促有丝分裂因子，由于其对间充质细胞的增殖和分化的作用，在组织工程中起着重要的作用。本研究的目的是探讨FGF-2在AMC软骨分化中的功能及其对骨组织再生中基于细胞的治疗的可能贡献。结果表明，FGF-2能显著促进AMC的增殖，并增强三维微团培养中的软骨形成。此外，用10 ng/ml的FGF-2处理引发AMC表明细胞经历软骨形成表型分化，这可能是通过诱导N-钙粘蛋白、FGF-受体2和转录因子Sox 9。我们的研究结果表明，FGF-2增强AMC中的软骨形成，类似于其在BMSCs中的功能，这表明FGF-2在骨骼再生和软骨修复中的多方面潜在应用。(c)2006年爱思唯尔公司All rights reserved.
Adipose-derived mesenchymal cells (AMCs) have demonstrated a great capacity for differentiating into bone, cartilage, and fat. Studies using bone marrow-derived mesenchymal cells (BMSCs) have shown that fibroblast growth factor (FGF)-2, a potent mitogenic factor, plays an important role in tissue engineering due to its effects in proliferation and differentiation for mesenchymal cells. The aim of this study was to investigate the function of FGF-2 in AMC chondrogenic differentiation and its possible contributions to cell-based therapeutics in skeletal tissue regeneration. Data demonstrated that FGF-2 significantly promoted the proliferation of AMCs and enhanced chondrogenesis in three-dimensional micromass culture. Moreover, priming AMCs with treatment of FGF-2 at 10 ng/ml demonstrated that Cells underwent chondrogenic phenotypic differentiation, possibly by inducing N-Cadherin, FGF-receptor 2, and transcription factor Sox9. Our results indicated that FGF-2 potentiates chondrogenesis in AMCs, similar to its functions in BMSCs, suggesting the versatile potential applications of FGF-2 in skeletal regeneration and cartilage repair. (c) 2006 Elsevier Inc. All rights reserved.