Insecticide resistance and cross-resistance in Alabama and Florida strains of Culex quinquefaciatus
Insecticide resistance and cross-resistance in Alabama and Florida strains of Culex quinquefaciatus
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DOI：
10.1603/0022-2585-41.3.408
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发表时间：
2004-05-01
影响因子：
2.1
通讯作者：
Liu, NN
中科院分区：
文献类型：
--
作者：
Liu, HQ;Cupp, EW;Liu, NN
在美国东南部测定了三个致倦库蚊品系的抗药性和交叉抗药性。HAmCq和MAmCq菌株于2002年从亚利桑那州亨茨维尔和莫比尔收集。VBFmCq株系于1998年从佛罗里达州韦罗比奇采集。与S实验室敏感品系相比，VBFmCq、HAmCq和MAmCq幼虫对氯氰菊酯表现出抗性，抗性倍数分别为13、100和940。HAmCq和MAmCq幼虫对氯氰菊酯的抗性分别为200倍和830倍，对马拉硫磷的抗性分别为4倍和70倍。VBFmCq、HAmCq和MAmCq菌株均表现出对不同杀虫剂的抗性和/或交叉抗药性，包括溴氰菊酯(50、100和300倍)、毒死蜱(150、33和720倍)、氟虫腈(10、5和15倍)和吡虫啉(分别为7.5、5和10倍)。对拟除虫菊酯、有机磷和吡虫啉的抗性比率的LC_(50)和LC_(90)以及剂量-反应曲线的渐变斜率的比较表明，VBFmCq、HAmCq和MAmCq对这些杀虫剂的反应是杂合性的。这三个菌株对苏云金芽孢杆菌以色列变种(Bti)和多杀菌素都表现出高度的敏感性，尽管这些蚊子已经广泛接触Bti。因此，我们认为Bti和Spinosad可能对CX的治疗有价值。尤其是在当地菌株对其他杀虫剂具有高度抗药性的情况下。
Insecticide resistance and cross-resistance was determined for three strains of Culex quinquefasciatus Say in the southeastern United States. HAmCq and MAmCq strains were collected in 2002 from Huntsville and Mobile, AL. The VBFmCq strain was collected in 1998 from Vero Beach, FL. VBFmCq, HAmCq, and MAmCq larvae showed resistance to permethrin with resistance ratios of 13, 100, and 940, respectively, compared with the susceptible S-Lab strain. Levels of resistance in HAmCq and MAmCq larvae were 200- and 830-fold to resmethrin and 4- and 70-fold to malathion, respectively. VBFmCq, HAmCq, and MAmCq strains all demonstrated a great ability to develop tolerance and/or cross-resistance to different insecticides, including deltamethrin (50-, 100-, and 300-fold), chlorpyrifos (150-, 33-, and 720-fold), fipronil (10-, 5-, and 15-fold), and imidacloprid (7.5-, 5- and 10-fold, respectively). Comparison of resistance ratios for pyrethroids, organ ophosphates, and imidacloprid at LC50 and LC90 and gradual slopes of dose-response curves indicated that VBFmCq, HAmCq, and MAmCq were heterozygous in response to these insecticides. All three strains showed high levels of susceptibility to Bacillus thuringiensis variety israelensis (Bti) and spinosad, although these mosquitoes had been extensively exposed to Bti. Thus, we conclude that Bti and spinosad may be valuable for the management of Cx. quinquefasciatus, especially in situations where local strains are highly resistant to other insecticides.