COMPARISON OF CYTOKERATIN, FILAGGRIN AND INVOLUCRIN PROFILES IN ORAL LEUKOPLAKIAS AND SQUAMOUS CARCINOMAS
COMPARISON OF CYTOKERATIN, FILAGGRIN AND INVOLUCRIN PROFILES IN ORAL LEUKOPLAKIAS AND SQUAMOUS CARCINOMAS
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DOI：
10.1111/j.1600-0714.1989.tb01569.x
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发表时间：
1989-08-01
影响因子：
3.3
通讯作者：
HANEKE, E
中科院分区：
文献类型：
--
作者：
VIGNESWARAN, N;PETERS, KP;HANEKE, E
由于细胞角蛋白 (CK)、聚丝蛋白和外皮蛋白的分布模式最近被建议区分良性和恶性上皮生长，因此使用一组针对不同细胞角蛋白多肽（34.β.E12、KL1 和 Pkk1）的 4 种单克隆抗体 (AB) 进行免疫组织化学检查，对健康口腔粘膜活检、不伴有不典型增生和不伴有不典型增生的白斑以及鳞状细胞癌进行免疫组织化学检查。聚丝蛋白以及外皮蛋白的多克隆 AB。在不伴上皮不典型增生和伴上皮不典型增生的白斑之间，观察到 CK（Pkk1 除外）、聚丝蛋白和外皮蛋白的分布存在重大且统计学上显着的差异。然而，CKs、丝聚蛋白和外皮蛋白表达的改变被证明并不是不典型增生的白斑中的恒定特征，因为其中相当一部分（20-25%）显示出与良性白斑相似的 CKs、丝聚蛋白和外皮蛋白的特征，反之亦然。迄今为止，这些抗原的免疫染色并不能比传统组织学的分级更准确地定义白斑异常增生的诊断。然而，免疫组织化学敏感性检测不典型增生白斑中角质形成细胞异常成熟模式的广泛变异，可用于将病变分为亚组，以在后续研究中阐明其预后。
As the distribution pattern of cytokeratin (CK), filaggrin and involucrin ahs recently been suggested to discriminate between benign and malignant epithelial growths, biopsies of healthy oral mucosa, leukoplakias without and with dysplasia and squamous cell carcinomas were examined immunohistochemically using a panel of 4 monoclonal antibodies (AB) against different cytokeratin polypeptides (34.beta.E12, KL1 and Pkk1) and filaggrin as well as a polyclonal AB to involucrin. Major and statistically significant differences were observed in the profiles of CKs (except Pkk1), filaggrin and involucrin between leukoplakias without and with epithelial dysplasia. However, the alteration in the expression of CKs, filaggrin and involucrin proved to be not a constant feature in leukoplakias with dysplasia as a considerable portion (20-25%) of them revealed the profiles of CKs, filaggrin and involucrin similar to those of benign leukoplakias, and vice versa. Immunostaining of these antigens did not define the diagnosis of dysplasia in leukoplakias more precisely than grading in conventional histology can do so far. However, immunohistochemical sensitivity in detecting a broad range of variation in the abnormal maturation patterns of keratinocytes in leukoplakias with dysplasia can be used to divide that lesions into subgroups to elucidate their prognosis in follow-up studies.