Effects of experimental periodontitis on the metabolic system in rats with diet-induced obesity (DIO): an analysis of serum biochemical parameters
Effects of experimental periodontitis on the metabolic system in rats with diet-induced obesity (DIO): an analysis of serum biochemical parameters
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DOI：
10.1007/s10266-017-0322-5
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发表时间：
2018-04-01
期刊：
影响因子：
2.5
通讯作者：
Numabe, Yukihiro
中科院分区：
文献类型：
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作者：
Kuraji, Ryutaro;Fujita, Miyako;Numabe, Yukihiro
最近的研究表明，牙周炎加速了肥胖相关代谢性疾病的进展。因此，我们研究了牙周炎对饮食诱导的肥胖（DIO）大鼠代谢性疾病的血清生化参数的影响。首先，我们使用10只雄性大鼠建立DIO模型，分别给予基础饮食（瘦肉组）和高脂饮食（DIO组）12周。其次，为了研究牙周炎和肥胖之间的相互作用，我们将24只DIO大鼠分为以下四组。(1)牙龈卟啉单胞菌（Pg）组在上颌第一磨牙（M1）周围涂抹Pg。(2)结扎组在M1周围进行结扎。(3)结扎/Pg组同时进行结扎和Pg治疗。（4）对照组为非治疗组。在12周时评价血清生化指标和上颌骨组织病理学。DIO模型显示，与瘦型组相比，体重、血清胰岛素、丙氨酸氨基转移酶（ALT）水平和胰岛素抵抗（HOMA-IR）显著增加。在DIO结扎和结扎/Pg组中，与对照组相比，牙槽骨吸收和炎性细胞浸润显著增加。空腹血糖、乳酸脱氢酶和尿酸的血清水平也显著较高，而肝损伤标志物ALT和天冬氨酸转氨酶仅在结扎/Pg组中较高。然而，我们观察到Pg组和对照组之间没有显著差异。本研究提示结扎引起的牙周炎可能改变了DIO大鼠肝脏等器官的血清代谢参数。
Recent studies have shown that periodontitis accelerates the progression of obesity-associated metabolic diseases. Thus, we examined the influence of periodontitis on serum biochemical parameters of metabolic disease in a diet-induced obesity (DIO) rat. First, we established the DIO model using ten male rats fed with either basal diet (lean group) or high-fat diet (DIO group) for 12 weeks. Second, to examine the interaction between periodontitis and obesity, we divided 24 DIO rats into the following four groups. (1) Porphyromonas gingivalis (Pg) group was applied with Pg around the maxillary first molar (M1). (2) Ligature group was applied with ligature placement around M1. (3) Ligature/Pg group was treated with both ligature placement and Pg. (4) Control was non-treatment group. Serum biochemical parameters and maxillary histopathology were evaluated at 12 weeks. The DIO model demonstrated significant increases in body weight, serum insulin, alanine aminotransaminase (ALT) levels, and insulin resistance (HOMA-IR) compared to the lean group. In the DIO ligature and ligature/Pg groups, alveolar bone resorption and inflammatory cell infiltration were significantly increased compared to the control. Serum levels of fasting glucose, lactate dehydrogenase, and uric acid were also significantly higher, while the liver damage markers ALT and aspartate aminotransferase were only higher in ligature/Pg group. However, we observed no significant differences between the Pg group and Control. The present study suggested a possibility that periodontitis induced by ligature placement changed serum metabolic parameter regarding organs such as the liver in DIO rat.