Endocochlear potential and endolymphatic K+ changes induced by gap junction blockers
Endocochlear potential and endolymphatic K+ changes induced by gap junction blockers
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DOI：
10.1080/00016480410017369
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发表时间：
2004-10-01
影响因子：
1.4
通讯作者：
Ikeda, K
中科院分区：
文献类型：
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作者：
Suzuki, M;Kikuchi, T;Ikeda, K
目的：探讨间隙连接阻滞剂对耳蜗电位（EP）和内淋巴钾浓度（[K+](e)）的影响。材料和方法：采用双管离子选择微电极在豚鼠耳蜗第二圈淋巴周围灌注时监测EP和[K+](e)。结果：用含有10 mM正庚醇的人工淋巴管灌注耳蜗淋巴周围鳞片时，EP下降- 8.8 +/- 1.4 mV (n = 10), [K+](e)下降-6.7 +/- 2.1 mM （n = 6）。淋巴周围应用10 mM己醇也导致EP和[K+]下降(e)。在对照研究中，淋巴周围灌注10mm乙醇，EP和[K+]均无显著变化(e)。与对照组类似，庚醇灌注期间缺氧导致EP呈负变化。结论：作为间隙连接阻滞剂的长链正烷醇引起EP下降并降低[K+](e)，可能是由于与间隙连接功能障碍相关的钾离子运输中断。
Objective - To examine the effects of gap junction blockers on the endocochlear potential (EP) and endolymphatic potassium concentration ([K+](e)).Material and Methods - The EP and [K+](e) were monitored using double-barreled ion-selective microelectrodes in the second turn of the guinea pig cochlea during perilymphatic perfusion.Results - When the perilymphatic scalae of the cochlea were perfused with artificial perilymph containing 10 mM n-heptanol the EP was decreased by - 8.8 +/- 1.4 mV (n = 10), and this was accompanied by a decline in the [K+](e) of -6.7 +/- 2.1 mM (n = 6). Perilymphatic application of 10 mM hexanol also produced declines in both the EP and [K+](e). In control studies, perilymphatic perfusion with 10 mM ethanol showed no remarkable changes in either the EP or [K+](e). Anoxia during perfusion with heptanol resulted in the generation of a negative EP, similar to the situation in controls.Conclusions - A decline in the EP together with a lowering of [K+](e) induced by long-chain n-alkanols, which act as gap junction blockers, may be explained by an interruption in potassium ion transport related to a gap junction dysfunction.