Nanostructured Si-nanowire microarrays for enhanced-performance bio-analytics
Nanostructured Si-nanowire microarrays for enhanced-performance bio-analytics
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DOI：
10.1039/c2lc40673j
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Choi, W. K.
中科院分区：
文献类型：
--
作者：
Dawood, M. K.;Zhou, L.;Choi, W. K.
我们展示了一种基于硅纳米线阵列的新型平台的制造，该平台集成了可编程dna定向均相分析物捕获策略，用于生物分析物的稳健检测。所使用的纳米制造工艺，基于扫角沉积和金属辅助催化蚀刻的结合，能够在每个芯片上产生数千个测试点，并且可以在整个晶圆上制造这些点，使用传统的微电子技术精确控制尺寸和定位。所使用的分析物捕获策略消除了众所周知的非均相（底物）与捕获分析物的干扰。我们研究了衬底的纳米尺度特征（纳米线孔隙度和团块）对分析物耦合效率的影响，并表明制备的微阵列具有鲁棒性，高效率和高容量，并提供显着增强的信噪比检测。
We demonstrate the fabrication of a novel platform based on Si nanowire arrays integrated with a programmable DNA-directed homogeneous-phase analyte-capture strategy for robust detection of bio-analytes. The nanofabrication process used, based on a combination of glancing-angle-deposition and metal-assisted-catalytic-etching, is capable of producing thousands of testing sites per chip, and the sites can be fabricated over entire wafers, with precise control of size and positioning, using conventional microelectronics technology. The analyte-capture strategy used eliminates the well-known interference of the heterogeneous-phase (substrate) with the capturing of analytes. We examine the effects of the nanoscale features of the substrates (nanowire porosity and clumping) on the coupling efficiency of analytes and show that the fabricated microarrays are robust, have high efficiency and capacity, and provide significantly enhanced signal-to-noise ratio detection.