Male courtship attractiveness and paternity success in Photinus greeni fireflies
Male courtship attractiveness and paternity success in Photinus greeni fireflies
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DOI：
10.1111/j.1558-5646.2007.00033.x
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发表时间：
2007-02-01
期刊：
影响因子：
3.3
通讯作者：
Lewis, Sara M.
中科院分区：
文献类型：
--
作者：
Demary, Kristian C.;Lewis, Sara M.
尽管女性择偶和男性精子竞争分别引起了很多关注，但很少有研究涉及交配前和交配后的性选择如何相互作用来驱动男性特征的进化。在石楠属萤火虫中，雌性根据其生物发光的求爱信号优先对雄性做出反应，雌性通过在几个晚上的多次交配中获得雄性婚礼礼物来直接受益。我们在实验中操纵了青虫萤火虫的交配，以检验交配后的父子关系成功可能偏向于在求偶互动中更具吸引力的雄性。我们首先使用现场行为分析来衡量男性对女性个体的求爱吸引力。雌性随后被分配到两个双重交配处理：(1)最不漂亮的第二个雄性--首先与最有吸引力的雄性交配，然后是最不漂亮的雄性，或者(2)最有吸引力的第二个雄性--以相反的顺序与雄性交配。利用随机扩增多态DNA(随机扩增多态性DNA)标记对每只雌蜂的后代进行了基因分型，并确定了父子关系。与预测相反，基于生物发光求偶表现而对雌性更具吸引力的萤火虫雄性随后表现出显著的低亲子关系，这反映了可能的雄性权衡或性冲突。男性父亲的差异与男性身体状况、睾丸或附腺质量无关，也与女性精囊大小的差异无关。此外，这项研究表明，表型选择梯度的变化可能发生在不同的生殖阶段。这些结果表明，将交配前和交配后的交配过程结合起来以充分理解雄性性状的进化，对于未来研究多雄同体物种的性别选择至关重要。
Although female mate choice and male sperm competition have separately attracted much attention, few studies have addressed how precopulatory and postcopulatory episodes of sexual selection might interact to drive the evolution of male traits. In Photinus fireflies, females preferentially respond to males based on their bioluminescent courtship signals, and females gain direct benefits through male nuptial gifts acquired during multiple matings over several nights. We experimentally manipulated matings of P.greeni fireflies to test the hypothesis that postcopulatory paternity success might be biased toward males that are more attractive during courtship interactions. We first measured male courtship attractiveness to individual females using field behavioral assays. Females were then assigned to two double-mating treatments: (1) least attractive second male-females were first mated with their most attractive male, followed by their least attractive male, or (2) most attractive second male-females mated with males in reverse order. Larval offspring produced by each female following these double matings were genotyped using random amplified polymorphic DNA (RAPD) markers, and male paternity was determined. Contrary to prediction, firefly males that were more attractive to females based on their bioluminescent courtship displays subsequently showed significantly lower paternity, reflecting possible male trade-offs or sexual conflict. Differences in male paternity were not related to male body condition, testes or accessory gland mass, or to variation in female spermathecal size. Additionally, this study suggests that changes in phenotypic selection gradients may occur during different reproductive stages. These results indicate that it is crucial for future studies on sexual selection in polyandrous species to integrate both precopulatory and postcopulatory episodes to fully understand the evolution of male traits.