Antinociceptive and Anti-Inflammatory Activities of Cuscuta chinensis Seeds in Mice
Antinociceptive and Anti-Inflammatory Activities of Cuscuta chinensis Seeds in Mice
复制标题
DOI：
10.1142/s0192415x14500153
复制
发表时间：
2014-01-01
影响因子：
5.7
通讯作者：
Peng, Wen-Huang
中科院分区：
文献类型：
--
作者：
Liao, Jung-Chun;Chang, Wen-Te;Peng, Wen-Huang
菟丝子（Cuscuta chinensis）的种子，即菟丝子（Cuscutae Semen），常用作治疗腰膝酸痛、补肝肾亏虚、治疗肾脾功能不全引起的腹泻的药材。由于疼痛和炎症与这些疾病高度相关，本研究的目的是探讨可能的镇痛和镇痛机制的种子。chinensis。结果表明，香蒲种子具有镇痛作用。通过醋酸诱导的扭体反应和福尔马林诱导的舔爪方法评价中国对虾。采用小鼠足跖肿胀法观察其抗炎作用。结果表明，100和500 mg/kg的川楝子种子甲醇提取物，在醋酸试验中，中华绒螯蟹（CCMeOH）显著降低（分别为p < 0：01和p < 0：001）扭体反应。此外，20-500 mg/kg的CCMeOH分别在福尔马林试验的早期（20和100 mg/kg，p < 0：001）和晚期（100 mg/kg，p < 0：01; 500 mg/kg，p < 0：001）显著减少舔体时间。此外，CCMeOH（100和500 mg/kg）在注射葡聚糖-角叉菜胶后4小时显著降低（分别为p < 0：01和p < 0：001）水肿爪体积。CCMeOH的抗炎作用机制可能是通过提高肝脏SOD、GPx和GRd的活性，降低足肿胀组织中NO和MDA的含量。此外，CCMeOH还降低了IL-1 β、IL-6、NF-κ B、TNF-α和考克斯-2水平。这是第一项证明CCMeOH体内抗伤害感受和镇痛作用的可能机制的研究。从而为菟丝子治疗炎症性疾病提供了依据。
The seeds of Cuscuta chinensis, Cuscutae Semen, are commonly used as a medicinal material for treating the aching and weakness of the loins and knees, tonifying the defects of the liver and the kidney, and treating the diarrhea due to hypofunction of the kidney and the spleen. Since aching and inflammation are highly correlated with such diseases, the aim of this study is to investigate the possible antinociceptive and antiinflammatory mechanisms of the seeds of C. chinensis. The antinociceptive effect of the seeds of C. chinensis was evaluated via the acetic acid-induced writhing response and formalin-induced paw licking methods. The anti-inflammatory effect was evaluated via the lambda-carrageenan induced mouse paw edema method. The results found that 100 and 500 mg/kg of the methanol extract of the seeds of C. chinensis (CCMeOH) significantly decreased (p < 0: 01 and p < 0: 001, respectively) the writhing response in the acetic acid assay. Additionally, 20-500 mg/kg of CCMeOH significantly decreased licking time at the early (20 and 100 mg/kg, p < 0: 001) and late phases (100 mg/kg, p < 0: 01; 500 mg/kg, p < 0: 001) of the formalin test, respectively. Furthermore, CCMeOH (100 and 500 mg/kg) significantly decreased (p < 0: 01 and p < 0: 001, respectively) edema paw volume four hours after lambda-carrageenan had been injected. The results in the following study also revealed that the anti-inflammatory mechanism of CCMeOH may be due to declined levels of NO and MDA in the edema paw by increasing the activities of SOD, GPx and GRd in the liver. In addition, CCMeOH also decreased IL-1 beta, IL-6, NF-kappa B, TNF-alpha, and COX-2 levels. This is the first study to demonstrate the possible mechanisms for the antinociceptive and antiinflammatory effects of CCMeOH in vivo. Thus, it provides evidence for the treatment of Cuscutae Semen in inflammatory diseases.