Round-cell mutants of Salmonella typhimurium produced by transposition mutagenesis: Lethality of rodA and mre mutations
Round-cell mutants of Salmonella typhimurium produced by transposition mutagenesis: Lethality of rodA and mre mutations
复制标题
通过转座诱变产生的鼠伤寒沙门氏菌圆细胞突变体：rodA 和 mre 突变的致死率
DOI：
10.1007/bf00277138
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
D. N. Antón
中科院分区：
文献类型：
--
作者：
C. Costa;D. N. Antón
在鼠伤寒沙门氏菌中分离到33个转座子Tn10Δl6Δl7插入到杆状细胞形状控制基因中，这些基因由球形细胞组成的菌落具有典型的光滑外观。基因检测显示25例（76%）为抗原插入，7例（21%）为突变体，1例（3%）为突变体。在pbpagene中未发现插入。细胞形状基因的插入只出现在对美西林耐药的菌株（不是由β-内酰胺酶产生的）。这两种菌株都不能转化为野生型菌株，但它们通常被耐美西林衍生物和细菌和突变体所接受，这些突变体与相应的大肠杆菌菌株不同，对美西林没有耐药性。另一方面，分割可以有效地转导到任何菌株。由此得出结论，RodA、mre和divd基因参与了杆状细胞形状的控制，但除此之外，RodA和mre产物还发挥了一些对野生型细胞必不可少的功能，而对一些耐美西林菌株、强霉素和突变体则是必不可少的。
Thirty-three insertions of transposon Tn10Δl6Δl7 into genes involved in the control of rod cell shape were isolated inSalmonella typhimuriumby the characteristic glossy appearance of colonies composed of spherical cells. Genetic tests demonstrated that 25 (76%) were insertions in therodAgene, 7 (21 %) weremremutants, and 1 (3%) was adivDmutant. No insertion in thepbpAgene were found. Insertions in cell shape genes only appeared when strains displaying resistance to mecillinam (not caused by β-lactamase production) were employed. NeitherrodAnormreinsertions could be transduced to wild-type strains but they were normally accepted by mecillinam-resistant derivatives and bycyaandcrpmutants, which, unlike the correspondingEscherichia colistrains, did not display resistance to mecillinam. On the other hand, thedivDinsertion could be efficiently transduced to any strain. It is concluded that therodA, mre, anddivDgenes are involved in the control of rod cell shape but, in addition, the RodA and Mre products perform some function(s) that is essential for wild-type cells but dispensable for some mecillinam-resistant strains, and forcyaandcrpmutants.