Increased expression of urotensin II and GPR14 in patients with cirrhosis and portal hypertension.
Increased expression of urotensin II and GPR14 in patients with cirrhosis and portal hypertension.
复制标题
肝硬化和门脉高压患者中尾加压素 II 和 GPR14 的表达增加。
DOI：
10.3892/ijmm_00000413
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发表时间：
2010
影响因子：
5.4
通讯作者：
Yu Wang
中科院分区：
文献类型：
--
作者：
Dian;Jing Chen;Jin Wang;Zhong;Xue;J. Jia;Yu Wang
尾加压素II（UII）及其受体（UT或GPR 14）参与肝纤维化和门脉高压。然而，UII/UT系统在门静脉高压症患者肝脏中的表达尚未阐明。采用实时荧光定量PCR技术，对乙型肝炎相关性肝硬化门脉高压症患者和正常对照组的肝活检标本中UII和UT基因表达进行定量。用免疫组化和免疫荧光法检测UT在肝脏的分布。Western印迹分析用于评估肝UT水平。同时，我们测量术中游离门静脉压（FPVP），并收集血浆用于ELISA法测定UII。肝硬化门脉高压症患者肝组织中UT mRNA和蛋白水平的表达均较健康对照组明显增强。UT蛋白表达主要集中在枯否细胞和肝窦内皮细胞。在肝硬化组织中，UII基因表达增加5倍，在正常肝组织相比。肝硬化患者血浆UII水平高于对照组，且与FPVP（r=0.807; P<0.001）和肝组织UII mRNA（r=0.802; P<0.001）相关。提示肝内UII/UT系统在肝硬化和门脉高压中具有重要的病理生理作用。
Urotensin II (UII) and its receptor (UT or GPR14) are involved in liver fibrosis and portal hypertension. Nevertheless, expression of the UII/UT system in the liver of patients with portal hypertension has not been elucidated. UII and UT gene expression were quantified in liver biopsy samples from patients with hepatitis-B-virus-associated cirrhosis and portal hypertension, and from normal controls by using quantitative real-time PCR. The liver distribution of UT was determined by means of immunohistochemistry and immunofluorescence. Western blot analysis was used to assess liver levels of UT. Simultaneously, we measured intra-operative free portal venous pressure (FPVP) and collected plasma for UII measurement by ELISA. UT expression at the mRNA and protein level was enhanced significantly in the liver of patients with cirrhosis and portal hypertension, compared with that in healthy controls. UT protein expression was concentrated mainly in the Kupffer cells and sinusoidal endothelial cells. In cirrhotic tissue, UII gene expression was increased 5-fold in comparison to that in normal liver tissue. Plasma UII level was higher in cirrhotic patients compared with controls and was correlated with FPVP (r=0.807; P<0.001) and UII mRNA in the liver (r=0.802; P<0.001). These findings suggest that the intrahepatic UII/UT system has an important pathophysiological role in cirrhosis and portal hypertension.
Urotensin II 是猪肾上皮细胞系 LLCPK1 的自分泌/旁分泌生长因子。
DOI：
--
发表时间：
2003
期刊：
Endocrinology 144
影响因子：
--
作者：
Matsushita M;Shichiri M;Fukai N;Ozawa N;Yoshimoto T;Takasu N;Hirata Y
通讯作者：
Hirata Y