A mouse model of intracerebral hemorrhage using autologous blood infusion
A mouse model of intracerebral hemorrhage using autologous blood infusion
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DOI：
10.1038/nprot.2007.513
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发表时间：
2008-01-01
期刊：
影响因子：
14.8
通讯作者：
Connolly, E. Sander
中科院分区：
文献类型：
--
作者：
Rynkowski, Michal A.;Kim, Grace H.;Connolly, E. Sander
建立可控、可重复的脑出血动物模型对于系统研究出血性卒中的病理生理和治疗至关重要。近年来，我们使用了一种改良的小鼠ICH模型，将血液注射到小鼠基底神经节中。根据我们的方案，将自体血液分两个阶段立体定向注入右侧纹状体，以模拟出血性卒中的自然事件。ICH诱导后，动物表现出可重现的血肿、脑水肿形成和显著的神经功能缺损。在许多急性和慢性研究中，我们的技术已被证明是在小鼠中创建ICH的可靠且可重现的方法。我们相信，我们的模型将作为一个理想的范式调查复杂的病理生理出血性中风。建立该模型的方案大约需要2h。
The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.