Critical roles of spo0A and spo0H in vegetative alkaline phosphatase production in Bacillus subtilis.
Critical roles of spo0A and spo0H in vegetative alkaline phosphatase production in Bacillus subtilis.
复制标题
spo0A 和 spo0H 在枯草芽孢杆菌营养性碱性磷酸酶生产中的关键作用。
DOI：
10.1128/jb.170.8.3765-3768.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
Jensen,K
中科院分区：
文献类型：
--
作者：
Hulett,FM;Jensen,K
为优化枯草芽孢杆菌中营养碱性磷酸酶的产生和稳定性而建立的生长条件用于比较等基因菌株 JH646 (spo0A12) 和 JH646MS (spo0A12 abrB15) 中碱性磷酸酶的合成和分泌。 spo0A 的突变阻止了植物碱性磷酸酶的产生，abrB 基因座的第二个突变导致植物碱性磷酸酶的过度诱导。在双突变体中，营养碱性磷酸酶合成的磷酸盐调节不受影响。多拷贝质粒上的 spo0H 部分克服了 spo0A 效应。
Growth conditions established to optimize vegetative alkaline phosphatase production and stability in Bacillus subtilis were used to compare alkaline phosphatase synthesis and secretion in isogenic strains JH646 (spo0A12) and JH646MS (spo0A12 abrB15). A mutation in spo0A blocked vegetative alkaline phosphatase production, and a second mutation at the abrB locus resulted in hyperinduction of vegetative alkaline phosphatase. Phosphate regulation of vegetative alkaline phosphatase synthesis was unaffected in the double mutant. spo0H, on a multicopy plasmid, partially overcame the spo0A effect.