IMMUNODEFICIENT CBA/N MICE RESPOND EFFECTIVELY TO CANDIDA-ALBICANS
IMMUNODEFICIENT CBA/N MICE RESPOND EFFECTIVELY TO CANDIDA-ALBICANS
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DOI：
10.1016/0090-1229(84)90308-8
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
DOMER, JE
中科院分区：
文献类型：
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作者：
CARROW, EW;HECTOR, RF;DOMER, JE
CBA/N小鼠中的免疫缺陷降低了它们对某些明确定义的抗原充分应答的能力。由于T细胞和B细胞在念珠菌病免疫中的作用尚未明确，因此希望CBA/N小鼠可以证明是研究对念珠菌特异性应答的有用模型。白色念珠菌免疫缺陷CBA/N和免疫活性CBA/J小鼠通过2次皮肤接种活的C.白色念珠菌B311，间隔2周接种，第2次接种后14天静脉内攻击。第2次接种后7天用膜衍生抗原（B-HEX）检测迟发型超敏反应（DTH），12天用B-HEX、胞质抗原（SCS）和促分裂原刺激淋巴细胞。在DTH试验后2天和静脉注射激发后28天，通过ELISA [酶联免疫吸附试验]测定SCS抗体，此时处死动物进行肾脏和脑定量培养。未处理CBA/N小鼠的平均对数集落形成单位（CFU）分别为3.79和5.48，并不比CBA/J小鼠更容易受到攻击。两种菌株对免疫的反应都是CFU的类似减少，即显著的DTH反应（例如，24小时的反应对于CBA/N为1.2mm，对于CBA/J为1.34mm），以及显著且相似量的抗体。CBA/N小鼠的免疫缺陷对C.白色念珠菌。
An immune defect in CBA/N mice diminishes their ability to respond adequately to certain well-defined antigens. Since the contribution of T and B cells to immunity in candidiasis has not been clearly defined, it was hoped that CBA/N mice might prove a useful model for the study of specific responses to C. albicans. Immunodeficient CBA/N and immunocompetent CBA/J mice were immunized by 2 cutaneous inoculations of viable C. albicans B311 given 2 wk apart and challenged i.v. 14 days after the 2nd inoculation. Delayed-type hypersensitivity (DTH) was tested with a membrane-derived antigen (B-HEX) 7 days following thte 2nd inoculation, and lymphocyte stimulation with B-HEX, a cytoplasmic antigen (SCS), and mitogens was done at 12 days. Antibody to SCS was determined by ELISA [enzyme-linked immunosorbent assay] 2 days after DTH testing and 28 days after i.v. challenge, at which time the animals were sacrificed for quantitative culture of kidneys and brains. Naive CBA/N mice were no more susceptible to challenge than CBA/J mice in that the mean log colony-forming units (CFU) were 3.79 and 5.48, respectively. Both strains responded to immunization by a similar reduction in CFU, a marked DTH response (e.g., reactions at 24 h were 1.2 mm for CBA/N and 1.34 mm for CBA/J), and significant and similar quantities of antibody. The immune defect in CBA/N mice had no demonstrable effect on the development of immune responses to infection with C. albicans.