Brassinosteroid's multi-modular interaction with the general stress network customizes stimulus-specific responses in Arabidopsis
Brassinosteroid's multi-modular interaction with the general stress network customizes stimulus-specific responses in Arabidopsis
复制标题
DOI：
10.1016/j.plantsci.2016.06.007
复制
发表时间：
2016-09-01
期刊：
影响因子：
5.2
通讯作者：
Dehesh, Katayoon
中科院分区：
文献类型：
--
作者：
Bjornson, Marta;Dandekar, Abhaya M.;Dehesh, Katayoon
适应波动的环境条件是植物生命的普遍特征，受优化资源分配的基本机制的支配。这种平衡部分是通过生长和应激反应信号通路之间严格调节的通信网络实现的。了解油菜素类固醇（BR），已知控制生长和胁迫适应的普遍存在的激素，和一般胁迫反应（GSR），响应扰动的快速和瞬时转录输出之间的通信模块，提供了一个最佳平台，以解开植物胁迫适应的新方面。在这里，我们探讨了通信方面的BR与GSR通过在植物中的GSR表达荧光素酶驱动的功能GSR顺式元件，快速应激反应元件（4xRSRE：LUC）的GSR定量。我们建立了外源性BR的应用抑制了微生物相关的分子模式激活的GSR，但增强了伤口触发的GSR。在BR处理的植物中，增强的伤口激活GSR导致更大的伤口诱导的对灰葡萄孢的抗性。使用BR信号突变体的分子遗传学和BR信号的激活剂比基尼的应用的组合，证实了这些结果，并将BR-GSR相互作用的主要点下游的潜在的膜受体电路。这些结果支持BR和胁迫信号之间的多模块相互作用，有助于定制拟南芥中的刺激特异性反应。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Adaptation to fluctuating environmental conditions is a universal feature of plant life, governed by fundamental mechanisms optimizing resource allocation. This balance is achieved in part through tightly regulated communication networks among growth and stress response signaling pathways. Understanding the communication modules between brassinosteroids (BRs), the ubiquitous hormones known to control growth and stress adaptation, and the general stress response (GSR), a rapid and transient transcriptional output in response to perturbations, provides an optimal platform to unravel new facet(s) of plant stress adaptation. Here, we explore communication facets of BR with GSR via in planta quantification of the GSR in Arabidopsis expressing luciferase driven by a functional GSR cis-element, the Rapid Stress Response Element (4xRSRE:LUC). We establish that application of exogenous BR suppresses microbe associated molecular pattern-activated GSR, but enhances the wound-triggered GSR. The enhanced wound-activated GSR in BR-treated plants results in a greater wound-induced resistance to Botrytis cinerea. A combination of molecular genetics using BR signaling mutants and application of an activator of BR signaling, bikinin, confirms these results and places the chief point of BR-GSR interaction downstream of potential membrane receptor circuitry. These results support a multi-modular interaction between BRs and stress signaling, instrumental in customizing stimulus-specific responses in Arabidopsis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.