Induction of tenascin in healing wounds.
Induction of tenascin in healing wounds.
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DOI：
10.1083/jcb.107.6.2757
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发表时间：
1988-12
期刊：
影响因子：
--
通讯作者：
Liverani D
中科院分区：
文献类型：
--
作者：
Mackie EJ;Halfter W;Liverani D
应用免疫组织化学方法研究了细胞外基质糖蛋白tenascin在大鼠正常皮肤和愈合皮肤中的分布。在正常皮肤中，腱腱素分布稀疏，主要与基底膜相关。在创面中，皮肤各层次创面边缘张力蛋白的表达均显著增加。在迁移、增殖表皮下的真皮-表皮连接处也有特别强的腱藤素染色。Tenascin存在于肉芽组织基质中，肉芽组织充满全层伤口，但在伤口收缩完成后的疤痕中未检测到。与纤连蛋白的分布在空间和时间上存在差异，在迁移表皮下，纤连蛋白出现在层粘连蛋白之前。Tenascin与肌成纤维细胞（收缩性伤口成纤维细胞）不完全共分布。在伤口的电镜研究中，tenascin定位于真皮-表皮交界处的基底层，以及相邻真皮基质的细胞外基质，在那里它要么均匀分布，要么与胶原纤维表面结合。在培养的皮肤外植体中，表皮在真皮层的切割边缘上迁移，在迁移上皮下面的真皮层中出现了张力蛋白，而不是纤维连接蛋白。这表明迁移、增殖的表皮诱导腱藤素的产生。本文的结果表明，腱藤素在伤口愈合中很重要，并且受到与纤维连接蛋白完全不同的调节机制的影响。
The distribution of the extracellular matrix glycoprotein, tenascin, in normal skin and healing skin wounds in rats, has been investigated by immunohistochemistry. In normal skin, tenascin was sparsely distributed, predominantly in association with basement membranes. In wounds, there was a marked increase in the expression of tenascin at the wound edge in all levels of the skin. There was also particularly strong tenascin staining at the dermal-epidermal junction beneath migrating, proliferating epidermis. Tenascin was present throughout the matrix of the granulation tissue, which filled full-thickness wounds, but was not detectable in the scar after wound contraction was complete. The distribution of tenascin was spatially and temporally different from that of fibronectin, and tenascin appeared before laminin beneath migrating epidermis. Tenascin was not entirely codistributed with myofibroblasts, the contractile wound fibroblasts. In EM studies of wounds, tenascin was localized in the basal lamina at the dermal-epidermal junction, as well as in the extracellular matrix of the adjacent dermal stroma, where it was either distributed homogeneously or bound to the surface of collagen fibers. In cultured skin explants, in which epidermis migrated over the cut edge of the dermis, tenascin, but not fibronectin, appeared in the dermis underlying the migrating epithelium. This demonstrates that migrating, proliferating epidermis induces the production of tenascin. The results presented here suggest that tenascin is important in wound healing and is subject to quite different regulatory mechanisms than is fibronectin.