Impact of intensive venous sampling on characterization of pulsatile GH release.
Impact of intensive venous sampling on characterization of pulsatile GH release.
复制标题
密集静脉采样对脉动 GH 释放特征的影响。
DOI：
10.1152/ajpendo.1987.252.4.e549
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Thorner,MO
中科院分区：
文献类型：
--
作者：
Evans,WS;Faria,AC;Christiansen,E;Ho,KY;Weiss,J;Rogol,AD;Johnson,ML;Blizzard,RM;Veldhuis,JD;Thorner,MO
使用客观的、基于统计的脉冲检测算法（聚类）测试采样强度对脉冲式生长激素（GH）释放的定量特性的影响。7名正常青年男子每隔5分钟抽血24小时。GH峰数/24 h在5分钟系列中检测到的（平均值+/- SE）（5.93 +/- 0.66）显著大于组分10分钟内记录的峰数（3.45 +/- 0.28）、15-min（2.79 +/- 0.31）、20-min（2.86 +/- 0.64）、30-min（2.5 +/- 0.36）、45-min（2.21 +/- 0.21）和60-min（1.93 +/- 0.23）系列。5分钟采样检测到的峰数增加反映了主要GH分泌期内发生的高频脉动GH释放。与5分钟系列中确定的峰相关的平均宽度和面积均小于采用较低强度采样记录的值。峰值振幅不随采样强度变化。这些数据表明，在正常的年轻男性GH释放的主要分泌期包括高频率的GH分泌活动。这些高频GH分泌事件之前尚未被描述过，这可能反映了相对不频繁的采样范式（例如，每20-30分钟一次）。
The effect of sampling intensity on quantitative properties of pulsatile growth hormone (GH) release was tested using an objective, statistically based pulse detection algorithm (Cluster). Seven normal young men had blood withdrawn at 5-min intervals for 24 h. The number of GH peaks/24 h (mean +/- SE) detected in the 5-min series (5.93 +/- 0.66) was significantly greater than the number of peaks documented in the constituent 10-min (3.45 +/- 0.28), 15-min (2.79 +/- 0.31), 20-min (2.86 +/- 0.64), 30-min (2.5 +/- 0.36), 45-min (2.21 +/- 0.21), and 60-min (1.93 +/- 0.23) series. The increased number of peaks detected with 5-min sampling reflected high-frequency pulsatile GH release occurring within the major GH secretory episodes. Both the mean widths and areas associated with peaks identified in the 5-min series were smaller than those documented with less intensive sampling. Peak amplitude did not change with sampling intensity. These data suggest that the major secretory episodes of GH release in normal young men encompass high-frequency GH secretory activity. That these high-frequency GH secretory events have not previously been described probably reflects the relatively infrequent sampling paradigms (e.g., every 20-30 min) commonly used in the past.