Molecular Typing of Treponema pallidum: a 5-Year Surveillance in Shanghai, China
Molecular Typing of Treponema pallidum: a 5-Year Surveillance in Shanghai, China
复制标题
DOI：
10.1128/jcm.01195-12
复制
发表时间：
2012-11-01
影响因子：
9.4
通讯作者：
Zhou, Pingyu
中科院分区：
文献类型：
--
作者：
Dai, Ting;Li, Kang;Zhou, Pingyu
此前，一项小型研究表明，14 f是中国上海梅毒螺旋体的主要亚型。这一结果与中国其他地区的基因型分布有很大不同。本研究旨在对上海市5年来采集的梅毒螺旋体标本进行分型。从2007年到2011年，收集了来自上海皮肤病医院的梅毒患者的生殖器拭子。通过在现有的阿普和tpr基因型系统中添加tp 0548基因，采用增强型分型方法对阳性标本进行分型。在372名入组患者中，共有304名患者产生了完全可分型的DNA。鉴定出10种阿普型（4、6、8、9、11、12、13、14、15和19）、3种TPR型（a、d和o）和5种TP 0548型（a、c、f、g和i）。总共有12种亚型被鉴定为阿普和tpr基因的组合。14 d亚型270例（88.8%）。当该组合包括tp 0548基因时，鉴定的12种CDC亚型被分为14种菌株类型。以14 d/f（88.8%）为主，其次为15 d/f（3.6%）、13 d/f（1.3%）和19 d/c（1.3%）。44例14 d/f感染患者中的2例和2例接受腰椎穿刺的19 d/c感染患者被诊断为神经梅毒。结果表明，上海地区以14 d/f为优势型。虽然这与中国其他地区的数据一致，但与早期报告显示14 f是上海最常见的基因型不同。需要进一步的研究来更好地了解菌株类型和神经梅毒之间的关联。
Previously, a small study showed that 14f was the predominant subtype of Treponema pallidum in Shanghai, China. The result was quite different from the genotype distribution in other areas of China. This study aimed to identify the strain types of Treponema pallidum in samples collected over a 5-year period in Shanghai. From 2007 to 2011, genital swabs were collected from patients with syphilis from the Shanghai Skin Disease Hospital. Positive specimens were typed by the enhanced typing method by adding a tp0548 gene to the existing arp and tpr genotype system. In total, 304 of the 372 enrolled patients yielded fully typeable DNA. Ten arp types (4, 6, 8, 9, 11, 12, 13, 14, 15, and 19), 3 tpr types (a, d, and o), and 5 tp0548 types (a, c, f, g, and i) were identified. In total, 12 subtypes were identified with a combination of the arp and tpr genes. Subtype 14d was found in 270 samples (88.8%). When the combination included the tp0548 gene, the 12 CDC subtypes identified were divided into 14 strain types. The predominant type was 14d/f (88.8%), followed by 15d/f (3.6%), 13d/f (1.3%), and 19d/c (1.3%). Two of the 44 14d/f-infected patients and both of the 19d/c-infected patients who underwent a lumbar puncture were diagnosed with neurosyphilis. This study showed that the predominant type in Shanghai was 14d/f. While this is in keeping with data from other areas in China, it is different from an earlier report showing that 14f is the most common genotype in Shanghai. Further studies are needed to better understand the association between strain types and neurosyphilis.