Altered expression of the PTR/NRT1 homologue OsPTR9 affects nitrogen utilization efficiency, growth and grain yield in rice
Altered expression of the PTR/NRT1 homologue OsPTR9 affects nitrogen utilization efficiency, growth and grain yield in rice
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DOI：
10.1111/pbi.12031
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发表时间：
2013-05-01
影响因子：
13.8
通讯作者：
Zhang, Mingyong
中科院分区：
文献类型：
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作者：
Fang, Zhongming;Xia, Kuaifei;Zhang, Mingyong
植物PTR/NRT1(肽转运体/硝酸盐转运体1)基因家族包括二/三肽和低亲和力硝酸盐转运体；一些成员还识别其他底物，如羧酸盐、植物激素(生长素和脱落酸)或防御化合物(硫代葡萄糖苷)。关于水稻(Oryza sativa L.)这个基因家族的成员，人们知之甚少。在此，我们报道了OsPTR9基因表达的改变对氮素利用效率、生长和产量的影响。OsPTR9的表达受外源氮素和昼夜循环的调控。转OsPTR9基因水稻植株中表达的OsPTR9增加了对氨的吸收，促进了侧根的形成，提高了产量。另一方面，OsPTR9在T-DNA插入系(Osptr9)和OsPTR9-RNAi植株中的下调则具有相反的效果。这些结果表明，OsPTR9在水稻育种中可能具有提高氮素利用率和提高产量的潜力。
The plant PTR/NRT1 (peptide transporter/nitrate transporter 1) gene family comprises di/tripeptide and low-affinity nitrate transporters; some members also recognize other substrates such as carboxylates, phytohormones (auxin and abscisic acid), or defence compounds (glucosinolates). Little is known about the members of this gene family in rice (Oryza sativa L.). Here, we report the influence of altered OsPTR9 expression on nitrogen utilization efficiency, growth, and grain yield. OsPTR9 expression is regulated by exogenous nitrogen and by the day-night cycle. Elevated expression of OsPTR9 in transgenic rice plants resulted in enhanced ammonium uptake, promotion of lateral root formation and increased grain yield. On the other hand, down-regulation of OsPTR9 in a T-DNA insertion line (osptr9) and in OsPTR9-RNAi rice plants had the opposite effect. These results suggest that OsPTR9 might hold potential for improving nitrogen utilization efficiency and grain yield in rice breeding.