One-signal requirement for interferon-gamma production by human large granular lymphocytes.
One-signal requirement for interferon-gamma production by human large granular lymphocytes.
复制标题
人大颗粒淋巴细胞产生干扰素-γ 的单一信号要求。
作者：
H. Young;J. Ortaldo
本实验室一直在研究高纯度人大颗粒淋巴细胞（LGL）和T细胞的IFN-γ基因表达。我们在这里报告，白细胞介素2（IL 2）处理后1小时内新鲜分离的人LGL，IFN-γ mRNA可以检测到，IFN-γ蛋白在4至6小时的治疗培养基中。CD 3- Leu-11+ LGL产生IFN-γ仅需要单一信号，因为植物血凝素（PHA）、佛波醇肉豆蔻酸酯乙酸酯（PMA）、IL 2或离子霉素可各自独立地诱导IFN-γ产生。此外，PHA和离子霉素（而不是IL 2）显示出显着的协同作用与PMA作为刺激LGL。相比之下，CD 3 + T细胞需要两种刺激来产生高水平的IFN-γ，并且不仅PMA加离子霉素或PHA具有协同作用，而且IL 2和PHA也表现出一定的协同作用。此外，我们通过外周血淋巴细胞的分级分离发现，IL 2诱导的IFN-γ产生与LGL群体而不是T细胞相关。这些结果表明，在某些刺激下，LGL可能是来自外周血淋巴细胞的IFN-γ的主要来源。
This laboratory has been investigating IFN-gamma gene expression by highly purified human large granular lymphocytes (LGL) and T cells. We report here that within 1 hr after interleukin 2 (IL 2) treatment of freshly isolated human LGL, IFN-gamma mRNA can be detected, with IFN-gamma protein in the culture medium within 4 to 6 hr of treatment. CD3- Leu-11+ LGL require only a single signal for IFN-gamma production because phytohemagglutinin (PHA), phorbol myristate acetate (PMA), IL 2, or ionomycin can each independently induce IFN-gamma production. In addition, PHA and ionomycin (but not IL 2) show significant synergy with PMA as a stimulus to LGL. In contrast, CD3+ T cells require two stimuli for high levels of IFN-gamma production, and not only are PMA plus ionomycin or PHA synergistic, but in addition, IL 2 and PHA demonstrate some synergy. Furthermore, we have found by fractionation of peripheral blood lymphocytes that IL 2-induced IFN-gamma production is associated with the LGL population and not T cells. These results indicate that with certain stimuli, LGL may be the predominant source of IFN-gamma from peripheral blood lymphocytes.