The abundance of milk cathelicidin proteins during bovine mastitis
The abundance of milk cathelicidin proteins during bovine mastitis
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DOI：
10.1016/j.vetimm.2011.06.034
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发表时间：
2011-09-15
影响因子：
1.8
通讯作者：
Haigh, B. J.
中科院分区：
文献类型：
--
作者：
Smolenski, G. A.;Wieliczko, R. J.;Haigh, B. J.
目前用于检测奶牛乳房炎的农场方法具有其特异性和灵敏度的局限性，特别是在感染的早期阶段。因此，有必要探索新的方法来检测早期和亚临床乳腺炎。本研究检测了一组嗜酸乳杆菌特异性蛋白质（cathelicidins）在自然发生的以及实验诱导的乳腺炎感染的乳汁样品中的表达。免疫印迹分析表明，cathelicidin蛋白质仅在感染的季度中观察到，并表现出高相关性与体细胞计数（SCC）在感染的发作。在检查的大多数感染中，cathelicidin在观察临床症状之前被检测到，并且SCC计数低至6.2 x 10（3）个细胞/mL。在自然发生的乳腺炎中，凯萨林菌素与感染状态之间的相关性并不强，25%的病原体阳性乳汁样品不含可检测的凯萨林菌素。这可能反映了在感染的不同阶段或严重程度时中性粒细胞浓度和活性的不同水平。我们的研究结果表明，牛奶cathelicidin水平增加乳房内感染和cathelicidin为基础的生物标志物可能有助于检测临床前乳腺炎或确定感染的阶段。(C)2011 Elsevier B. V.保留所有权利。
Current on-farm methods for detecting mastitis in dairy cows have limitations with their specificity and sensitivity, particularly at an early stage of infection. There is therefore a need to explore new approaches for detecting early and subclinical mastitis. This study examined the expression of a group of neutrophil-specific proteins, the cathelicidins, in milk samples from naturally occurring as well as experimentally induced mastitis infections. Immunoblot analysis indicated that cathelicidin proteins are only observed in infected quarters and demonstrate a high correlation with somatic cell count (SCC) during the onset of infection. In most of the infections examined, cathelicidin was detected prior to the observation of clinical symptoms and at SCC counts as low as 6.2 x 10(3) cells/mL. In naturally occurring mastitis the correlation between cathelicidin and infection status is not as strong, with 25% of pathogen-positive milk samples containing no detectable cathelicidin. This may reflect the varying levels of neutrophil concentration and activity at different stages or severities of infection. Our results indicate that milk cathelicidin levels increase following intramammary infection and cathelicidin-based biomarkers may assist in the detection of preclinical mastitis or determining the stage of infection. (C) 2011 Elsevier B.V. All rights reserved.