Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L.
Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L.
复制标题
供体株龄和花序龄对甘蓝型油菜小孢子培养的影响。
DOI：
10.1007/bf00035339
复制
发表时间：
1991
期刊：
影响因子：
1.9
通讯作者：
W. Keller
中科院分区：
文献类型：
--
作者：
Y. Takahata;D. C. Brown;W. Keller
供体植株年龄和花序年龄对白杨小孢子培养胚胎发生的影响。napuswas检查。从老植株的芽中分离出的小孢子的胚率高于幼植株。花序年龄的影响表现出不同的模式。在老植株中，从新花序芽中分离的小孢子具有较高的胚发生反应，而在幼植株中，从老花序芽中分离的小孢子具有较高的胚发生率。这些不同的反应被认为是由于小孢子分离时花粉发育阶段的不同。结果表明，在花粉的最佳发育阶段，从老花序和老植株中采集的小孢子具有足够的胚性。胚向植株转化的频率受继代胚大小的影响，而不受供体植株年龄和花序年龄的影响。
Effect of age of donor plants and age of inflorescence on embryogenesis in microspore culture ofB. napuswas examined. Microspores isolated from buds of older plants had a higher embryo yield than those of younger ones. The effect of the age of inflorescence showed a different pattern. In older plants, a higher embryogenesis response was observed in microspores isolated from buds of new inflorescences, while in young plants, microspores isolated from buds of old inflorescences showed high embryo yield. These different responses were considered to be attributable to a difference in the developmental stage of pollen at the time of microspore isolation. Our results indicated that microspores collected from older inflorescences and older plants have sufficient embryogenic potential when the optimum developmental stage of pollen was used. Frequency of embryo to plant conversion was influenced by the size of embryos subcultured, but not by donor plant age or the age of the inflorescence.