Flowering time modulation by a vacuolar SNARE via FLOWERING LOCUS C in Arabidopsis thaliana.
Flowering time modulation by a vacuolar SNARE via FLOWERING LOCUS C in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0042239
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ueda T
中科院分区：
文献类型：
--
作者：
Ebine K;Uemura T;Nakano A;Ueda T
植物从营养生长阶段向生殖生长阶段的转变是植物生活史中最重要和最引人注目的事件之一。在拟南芥中，开花促进涉及至少四个遗传定义的调节途径，包括光周期依赖性，春化依赖性，赤霉素依赖性和自主促进途径。在这些调节途径中，春化依赖性和自主性途径通过表达开花负调控基因座C（FLC）来整合;然而，该基因座的上游调控尚未完全了解。SYP 22基因编码一种在液泡和内吞运输途径中起作用的液泡SNARE蛋白。据报道，SYP 22功能的丧失导致A. thaliana植物，但其机制仍然完全未知。在这项研究中，我们证明，晚开花表型syp 22是由于FLC的自主途径受损引起的表达升高。此外，我们还研究了DOC 1/BIG通路，该通路也被认为调节液泡/内体运输。我们发现，FLC转录水平的升高积累在doc 1 -1突变体，和syp 22表型被夸大了双syp 22 doc 1 -1突变。我们进一步证明，FLC的表达升高受到ara 6 -1的抑制，ara 6 -1是编码植物特有的Rab GT3的基因中的突变，参与内体运输。我们的结果表明，液泡和/或内吞运输参与FLC调控开花时间在A。thaliana.
The transition of plant growth from vegetative to reproductive phases is one of the most important and dramatic events during the plant life cycle. In Arabidopsis thaliana, flowering promotion involves at least four genetically defined regulatory pathways, including the photoperiod-dependent, vernalization-dependent, gibberellin-dependent, and autonomous promotion pathways. Among these regulatory pathways, the vernalization-dependent and autonomous pathways are integrated by the expression of FLOWERING LOCUS C (FLC), a negative regulator of flowering; however, the upstream regulation of this locus has not been fully understood. The SYP22 gene encodes a vacuolar SNARE protein that acts in vacuolar and endocytic trafficking pathways. Loss of SYP22 function was reported to lead to late flowering in A. thaliana plants, but the mechanism has remained completely unknown. In this study, we demonstrated that the late flowering phenotype of syp22 was due to elevated expression of FLC caused by impairment of the autonomous pathway. In addition, we investigated the DOC1/BIG pathway, which is also suggested to regulate vacuolar/endosomal trafficking. We found that elevated levels of FLC transcripts accumulated in the doc1-1 mutant, and that syp22 phenotypes were exaggerated with a double syp22 doc1-1 mutation. We further demonstrated that the elevated expression of FLC was suppressed by ara6-1, a mutation in the gene encoding plant-unique Rab GTPase involved in endosomal trafficking. Our results indicated that vacuolar and/or endocytic trafficking is involved in the FLC regulation of flowering time in A. thaliana.
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