Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor
Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor
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DOI：
10.1177/154405910408300209
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发表时间：
2004-02-01
影响因子：
7.6
通讯作者：
Harada, H
中科院分区：
文献类型：
--
作者：
Kawano, S;Saito, M;Harada, H
牙上皮祖细胞在牙胚发育过程中分化成各种细胞类型。为了研究这一机制，我们从大鼠下切牙的颈环上皮中产生了永生化的牙齿上皮祖细胞。通过免疫组织化学检查较低和较高细胞密度中细胞角蛋白 14、神经生长因子受体 p75、牙釉蛋白、Notch2 和碱性磷酸酶的表达模式。在大鼠下切牙的牙上皮中比较了每种模式。结果表明，这些细胞可以产生成釉细胞谱系细胞、中间层细胞、星状网和外釉质上皮。此外，成纤维细胞生长因子 10 刺激牙齿祖细胞增殖，随后增加表达碱性磷酸酶的细胞数量。这些结果表明，成纤维细胞生长因子 10 在大鼠门牙中颈环细胞有丝分裂和中间层细胞产生的耦合中发挥作用。
Dental epithelial progenitor cells differentiate into various cell types during development of tooth germs. To study this mechanism, we produced immortalized dental epithelial progenitor cells derived from the cervical-loop epithelium of a rat lower incisor. The expression patterns of cytokeratin 14, nerve growth factor receptor p75, amelogenin, Notch2, and alkaline phosphatase were examined by immnohistochemistry in both lower and higher cell densities. The patterns of each were compared in the dental epithelium of rat lower incisors. The results demonstrated that these cells could produce ameloblast lineage cells, stratum intermedium cells, stellate reticulum, and outer enamel epithelium. Furthermore, fibroblast growth factor 10 stimulated proliferation of dental progenitor cells and subsequently increased the number of cells expressing alkaline phosphatase. These results suggest that fibroblast growth factor 10 plays a role in coupling mitogenesis of the cervical-loop cells and the production of stratum intermedium cells in rat incisors.